Cdk2 Monoclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with CDK2 monoclonal antibody, unconjugated (bsm-52027R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CDK2 monoclonal antibody, unconjugated (bsm-52027R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bsm-52027r
Product Name	Cdk2 Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Cdk2 protein, around C-terminal 100aa.
Clonality	Monoclonal
Clone #	2E3
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1017
Swiss Prot	P24941
Synonyms	CDKN2; p33(CDK2); Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; CDK2
Background	The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:300-5000