PLCG1 Recombinant Antibody

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Human A431 cell lysates; Lane 7: Human A549 cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human HeLa cell lysates probed with PLCG1 Monoclonal Antibody, Unconjugated (bsm-52174R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Human A431 cell lysates; Lane 7: Human A549 cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human HeLa cell lysates probed with PLCG1 Monoclonal Antibody, Unconjugated (bsm-52174R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-52174r
Product Name	PLCG1 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Phospholipase C gamma 1 protein, around C-terminal 150aa.
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5335
Swiss Prot	P19174
Synonyms	PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C-gamma-1; PLC-gamma-1; PLCG1
Background	Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC/IF	1:50-100