phospho-Src (Tyr419) Recombinant Antibody

Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.", "Category"=>"Primary Antibody", "Clonality"=>"Recombinant", "Concentration"=>"1ug/ul", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human, Mouse, Rat", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bsm-52208R", "Gene ID"=>"6714", "Gene ID Link"=>"Entrez Gene: 6714", "Host"=>"Rabbit", "images"=>"[{\"id\":41928,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":13332,\"productconjugation_id\":null,\"application_id\":1,\"application_note\":\"\",\"dilution\":\"1:1000\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Lane 1: Human Hela cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Jurkat cell lysates probed with phospho-Src (Tyr419) Monoclonal Antibody, Unconjugated (bsm-52208R) at 1:1000 dilution and 4\\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\",\"sample_type_id\":1,\"sample_details\":null,\"rating\":3,\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":23,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"13332\\/41928_raw.jpg\",\"filepath_med\":\"13332\\/41928_med.jpg\",\"filepath_thumb\":\"13332\\/41928_thumb.jpg\",\"display_order\":1,\"lock_version\":1,\"created_at\":\"2022-08-20 00:24:19\",\"updated_at\":\"2022-08-20 00:24:19\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/41928\"}]", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Phosphorylation", "Modification Site"=>"Y419", "Notes"=>"Hua-bio catalog number: ET1609-15. Website: http://www.huabio.com", "Product URL"=>"https://www.biossusa.com/products/bsm-52208R", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"Synthetic peptide derived from human Src(Y419), around 400-450aa (phospho Y419).", "Storage"=>"0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol Store at -20°C for 12 months.", "Storage Buffer"=>"0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol", "Storage Condition"=>"Store at -20°C for 12 months.", "Swiss Prot"=>"P12931", "Swiss Prot Link"=>"Swiss Prot: P12931", "Synonyms"=>"ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specic protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; Protooncogene SRC; protooncogene SRC Rous sarcoma; Src; SRC Oncogene; SRC_MOUSE; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC 1; Tyrosine protein kinase SRC1; v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog; V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian; v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian; vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog.", "Target Protein"=>"phospho-Src (Tyr419)"} -->