Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody

$Lane 1: Mouse testis; Lane 2: CRC lysate probed with Histone H3(mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse testis; Lane 2: CRC lysate probed with Histone H3(mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52291R
Product Name	Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide surrounding K79 of Histone H3
Modification Site	mono+di+methyl K79
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Swiss Prot	P68431
Synonyms	Histone H3, Histone H3
Background	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC