| Overview |
| bsm-52347r-bf350-100ul |
| PCNA Recombinant Antibody, AbBy Fluor-350 Conjugated |
| WB, IF, Flow-Cyt |
| Human, Mouse, Rat |
| Specifications |
| AbBy Fluor-350 |
| Rabbit |
| Recombinant human PCNA between 100 to 200 amino acids |
| Recombinant |
| IgG |
| Lot dependent |
| Purified by Protein A. |
| Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target |
| 5111 |
| P12004 |
| ProlifeRating cell nuclear antigen, Cyclin, PCNA |
| Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Application Dilution |
| WB |
1:300-5000 |
| IF |
|
| Flow-Cyt |
1ug/Test |
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