MAP1LC3A (1C7) Monoclonal Antibody

$Mouse Brain lysate probed with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Mouse Brain lysate probed with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100 in PMVEC cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.\r\n\r\n$

IF(ICC) staining with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100 in PMVEC cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.\r\n\r\n

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52595R
Product Name	MAP1LC3A (1C7) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant MAP1LC3A
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	84557
Swiss Prot	Q9H492
Synonyms	Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A
Background	Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC