Annexin V Recombinant Antibody

Flow cytometric analysis of HeLa cells with Annexin V (6C7) Monoclonal Antibody (bsm-52841R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$IF(ICC) staining with Annexin V (6C7) Monoclonal Antibody (bsm-52841R) at 1:300 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Annexin V (6C7) Monoclonal Antibody (bsm-52841R) at 1:300 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Lane 1: Mouse Lung lysates; Lane 2: Mouse Placenta lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Spleen lysates; Lane 5: Mouse Stomach lysates; Lane 6: Rat Lung lysates; Lane 7: Rat Uterus lysates; Lane 8: Rat Spleen lysates; Lane 9: Rat Stomach lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human PANC-1 cell lysates; Lane 12: Human K562 cell lysates; Lane 13: Human Jurkat cell lysates; Lane 14: Human HUVEC cell lysates probed with Annexin V (6C7) Monoclonal Antibody, Unconjugated (bsm-52841R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52841r
Product Name	Annexin V Recombinant Antibody
Applications	WB, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Annexin V aa 1-150
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	308
Swiss Prot	P08758
Synonyms	Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CBP-I, PP4, PAP-I, VAC-alpha, ANXA5
Background	This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Application Dilution
WB	1:300-5000
Flow-Cyt	1ug/Test
ICC/IF	1:50-100