ADAM10 Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM10 Monoclonal Antibody, Unconjugated(bsm-52935R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM10 Monoclonal Antibody, Unconjugated(bsm-52935R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with ADAM10 monoclonal antibody, unconjugated (bsm-52935R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ADAM10 monoclonal antibody, unconjugated (bsm-52935R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IP
IF

Reactivity

Human

Overview
Catalog #	bsm-52935r
Product Name	ADAM10 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human ADAM 10
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	102
Swiss Prot	O14672
Subcellular location	Cytoplasm, Cell membrane
Synonyms	A disintegrin and metalloprotease domain 10; A Disintegrin And Metalloproteinase Domain 10; ADAM 10; ADAM metallopeptidase domain 10; CD 156c; CD156c; CD156c antigen; CDw156; HsT 18717; HsT18717; kuz; kuzbanian; Kuzbanian protein homolog; MADM; Mammalian disintegrin metalloprotease; ADA10_HUMAN; RAK; kuz; AD10; AD18;
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF