LXR alpha Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with LXR alpha monoclonal antibody, unconjugated (bsm-54023R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with LXR alpha monoclonal antibody, unconjugated (bsm-54023R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54023r
Product Name	LXR alpha Recombinant Antibody
Applications	WB, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal Human LXR alpha
Immunogen Range	60-110/447
Clonality	Monoclonal
Clone #	4A8
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10062
Swiss Prot	Q13133
Subcellular location	Nucleus
Synonyms	Oxysterols receptor LXR-alpha; LXR alpha; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3; NR1H3; LXRA; RLD-1.
Background	Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism.
Application Dilution
WB	1:300-5000
Flow-Cyt	1ug/Test
ICC/IF	1:50-100