Flotillin 1 (4C3) Monoclonal Antibody

$Lane 1: PC-3M Cells; Probed with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: PC-3M Cells; Probed with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of HUVEC cells with Flotillin 1 (4C3) Monoclonal Antibody (bsm-54325R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54325R
Product Name	Flotillin 1 (4C3) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Flotillin 1 aa 200-400
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	10211
Swiss Prot	O75955
Subcellular location	Cell membrane, Endosome
Synonyms	Flotillin-1; FLOT1
Background	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC