UQCRFS1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with UQCRFS1 monoclonal antibody, unconjugated (bsm-54363R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with UQCRFS1 monoclonal antibody, unconjugated (bsm-54363R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54363r
Product Name	UQCRFS1 Recombinant Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human UQCRFS1 aa 100 to the C-terminus.
Clonality	Monoclonal
Clone #	10C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	7386
Swiss Prot	P47985
Subcellular location	Mitochondrion
Synonyms	Cytochrome b-c1 complex subunit Rieske mitochondrial; Complex III subunit 5; Cytochrome b-c1 complex subunit 5; Rieske iron-sulfur protein; Rieske protein UQCRFS1; Ubiquinol-cytochrome c reductase iron-sulfur subunit; Cytochrome b-c1 complex subunit 9; Complex III subunit IX; Cytochrome b-c1 complex subunit 11; Ubiquinol-cytochrome c reductase 8 kDa protein; RISP
Background	Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Incorporation of UQCRFS1 is the penultimate step in complex III assembly.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200