BOB1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated (bsm-54659R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated (bsm-54659R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of BOB1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54659R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of BOB1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54659R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human

Overview
Catalog #	bsm-54659R
Product Name	BOB1 Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within C-terminal Human BOB1.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5450
Swiss Prot	Q16633
Subcellular location	Nucleus
Synonyms	B cell Oct binding protein 1 antibody, B cell specific coactivator OBF 1 antibody, B cell specific coactivator OBF1 antibody, B-cell-specific coactivator OBF-1 antibody, BOB 1 antibody, BOB-1 antibody, OBF 1 antibody, OBF1 antibody, OBF1_HUMAN antibody, OCA B antibody, OCA-B antibody, OCAB antibody, OCT binding factor 1 antibody, OCT-binding factor 1 antibody, POU class 2 associating factor 1 antibody, POU domain class 2 associating factor 1 antibody, POU domain class 2-associating factor 1 antibody, Pou2af1 antibody
Background	This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC