PHB2 Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:10000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HeLa, 293, Mouse heart, Rat kidney\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 33 kDa\r\nObserved MW: 33 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:10000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HeLa, 293, Mouse heart, Rat kidney\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 33 kDa\r\nObserved MW: 33 kDa

Applications

WB
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60543r
Product Name	PHB2 Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Recombinant
Isotype	IgG/Kappa
Concentration	1mg/ml
Purification	Protein G.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	Q99623
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100