Histone H2A (Acetyl-Lys9) Monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103 overnight Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium\r\nbutyrate (50mM, 24hr) + trichostain A (500ng\/ml, 4 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 14 KDa Observed MW: 14 KDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103 overnight Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium\r\nbutyrate (50mM, 24hr) + trichostain A (500ng\/ml, 4 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 14 KDa Observed MW: 14 KDa

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103 overnight Secondary Ab: (-): NIH\/3T3, (+): NIH\/3T3+ trichostain A (1\u03bcM, 18 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 14 KDa Observed MW: 14 KDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103 overnight Secondary Ab: (-): NIH\/3T3, (+): NIH\/3T3+ trichostain A (1\u03bcM, 18 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 14 KDa Observed MW: 14 KDa

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-60663R
Product Name	Histone H2A (Acetyl-Lys9) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Histone H2A (Acetyl-Lys9)
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3012
Swiss Prot	Q16777
Subcellular location	Cytoplasm, Extracellular matrix
Synonyms	Acetyl-Histone H2A (Lys9); H2AK9ac; H2a 615; H2A; H2A GL101; H2A histone family member A; H2A.1; H2A.2; H2A/a; H2A/m; H2A/O; H2A/q; H2AFA; H2AFE; H2AFL; H2AFM; H2AFO; H2AFQ; HIST1H2AE; HIST1H2AJ antibody HIST2H2AA; HIST2H2AA3; HIST2H2AB; HIST2H2AC; Histone 1 H2ae; Histone 2 H2aa3; Histone 2 H2ab; Histone 2 H2ac; Histone H2A type 1 B; Histone H2A type 1 C; Histone H2A type 1 E; Histone H2A type 1 J; MGC74460.
Application Dilution
WB	1:300-5000