Phospho-AKT1S1 (Thr246) Recombinant Antibody

$NIH\/3T3 (M) cells were treated with or without insulin (150nM) for 15 min,Hela (H) cells were treated with or without insulin (100nM) for 5 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-AKT1S1 (Thr246) monoclonal antibody, unconjugated (bsm-61148R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

NIH\/3T3 (M) cells were treated with or without insulin (150nM) for 15 min,Hela (H) cells were treated with or without insulin (100nM) for 5 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-AKT1S1 (Thr246) monoclonal antibody, unconjugated (bsm-61148R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-61148r
Product Name	Phospho-AKT1S1 (Thr246) Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human AKT1S1 around the phosphorylation site of T246
Modification Site	Thr246
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84335
Swiss Prot	Q96B36
Subcellular location	Cytoplasm
Synonyms	AKT1S1; PRAS; PRAS40.
Background	Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
Application Dilution
WB	1:300-5000