ATG7 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with ATG7 monoclonal antibody, unconjugated (bsm-61217R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ATG7 monoclonal antibody, unconjugated (bsm-61217R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61217r
Product Name	ATG7 Recombinant Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATG7
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10533
Swiss Prot	O95352
Subcellular location	Cytoplasm
Synonyms	hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L.
Background	E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200