ABCF1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with ABCF1 monoclonal antibody, unconjugated (bsm-61283R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ABCF1 monoclonal antibody, unconjugated (bsm-61283R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human

Predicted Reactivity

Overview
Catalog #	bsm-61283r
Product Name	ABCF1 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ABCF1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23
Swiss Prot	Q8NE71
Subcellular location	Cytoplasm, Nucleus
Synonyms	ABC27; ABC50; ABCF1; ATP-binding cassette 50; ATP-binding cassette 50 (TNF-alpha stimulated); TNF-alpha-stimulated ABC protein.
Background	Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC/IF	1:50-100