ILK Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with ILK monoclonal antibody, unconjugated (bsm-61287R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ILK monoclonal antibody, unconjugated (bsm-61287R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IP
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61287r
Product Name	ILK Recombinant Antibody
Applications	WB, IP, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ILK
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3611
Swiss Prot	Q13418
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ILK; DKFZp686F1765; P59; ILK1; Integrin linked protein kinas.
Background	Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin.
Application Dilution
WB	1:300-5000
IP	1-2ug
Flow-Cyt	1ug/Test
ICC/IF	1:50-100