PCGF4/BMI1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PCGF4\/BMI1 monoclonal antibody, unconjugated (bsm-61372R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PCGF4\/BMI1 monoclonal antibody, unconjugated (bsm-61372R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Predicted Reactivity

Overview
Catalog #	bsm-61372r-TR
Product Name	PCGF4/BMI1 Recombinant Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BMI1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	100532731
Swiss Prot	P35226
Subcellular location	Cytoplasm, Nucleus
Synonyms	BMI1 ; Polycomb complex protein BMI 1; RING finger protein 51; RNF51; PCGF4.
Background	Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Application Dilution
WB	1:300-5000