ATG3 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with ATG3 monoclonal antibody, unconjugated (bsm-61474R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ATG3 monoclonal antibody, unconjugated (bsm-61474R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61474r
Product Name	ATG3 Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATG3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	64422
Swiss Prot	Q9NT62
Subcellular location	Cytoplasm
Synonyms	APG3 autophagy 3 like; APG3 like; APG3, S. cerevisiae, homolog of; APG3-like; APG3L; Apg3p; ATG3 autophagy related 3 homolog.
Background	E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation.
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100