PSGL-1/CD162 Recombinant Antibody

$4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (PSGL-1\/CD162) monoclonal Antibody, unconjugated (bsm-61840R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (PSGL-1\/CD162) monoclonal Antibody, unconjugated (bsm-61840R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$The Jurkat(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-PSGL-1\/CD162 antibody (bsm-61840R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Jurkat(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-PSGL-1\/CD162 antibody (bsm-61840R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

IHC-P
IHC-F
IP
IF
Flow-Cyt
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-61840r
Product Name	PSGL-1/CD162 Recombinant Antibody
Applications	IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PSGL 1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6404
Swiss Prot	Q14242
Synonyms	CD162; CLA; PSGL1; SELPLG.
Background	A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Application Dilution
IHC-P	WBWB=1:500-2000
IHC-F	IHC-PIHC-P=1:50-200
IP	IHC-FIHC-F=1:50-200
IF	IPIP=1:20-50
Flow-Cyt	IFIF=1:50-200
ICC/IF	Flow-CytFlow-Cyt=1ug/Test