TC10 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with TC10 monoclonal antibody, unconjugated (bsm-62046R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with TC10 monoclonal antibody, unconjugated (bsm-62046R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Human
Rat

Overview
Catalog #	bsm-62046r
Product Name	TC10 Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TC10
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23433
Swiss Prot	P17081
Synonyms	ARHQ; RASL7A; Rho related GTP binding protein RhoQ; Rhoq; TC10A.
Background	Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100