Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Antibody

$Western blot analysis of HeLa cell lysate. Using Mono\/Di\/TriMethyl-Histone H3.1 (Lys15) (bsm-62431R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HeLa cell lysate. Using Mono\/Di\/TriMethyl-Histone H3.1 (Lys15) (bsm-62431R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
ICC/IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-62431r
Product Name	Mono/Di/TriMethyl-Histone H3.1 (Lys15) Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Histone H3.1 around the methylation site of K15
Modification Site	Lys15
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8350
Swiss Prot	P68431
Subcellular location	Nucleus
Synonyms	H3 histone; HIST1H3A; Histone cluster 1, H3a;H3/l; HIST3H3; H3K14me1; H3K14me2; H3K14me3.
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100