Phospho-SHIP1 (Tyr1022) Recombinant Antibody

$Western blot analysis of (1) Raji cell lysate; (2) Raji cell treated with pervanadate lysate. Using Phospho-SHIP1 (Tyr1022) (bsm-62834R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) Raji cell lysate; (2) Raji cell treated with pervanadate lysate. Using Phospho-SHIP1 (Tyr1022) (bsm-62834R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IP
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-62834r
Product Name	Phospho-SHIP1 (Tyr1022) Recombinant Antibody
Applications	WB, IP, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human INPP5D around the phosphorylation site of Y1022
Modification Site	Tyr1022
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3635
Swiss Prot	Q92835
Synonyms	Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SHIP-1; p150Ship; hp51CN; INPP5D; SHIP.
Background	Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways.
Application Dilution
WB	1:300-5000
IP	IFICC1:50-200
ICC/IF	IP1-2ug