KDM1/LSD1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with KDM1\/LSD1 monoclonal antibody, unconjugated (bsm-62858R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with KDM1\/LSD1 monoclonal antibody, unconjugated (bsm-62858R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62858r-TR
Product Name	KDM1/LSD1 Recombinant Antibody
Applications	WB, IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KDM1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23028
Swiss Prot	O60341
Subcellular location	Nucleus
Synonyms	KDM1;AOF2;BHC110;KIAA0601;LSD1; KDM1A.
Background	Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
Application Dilution
WB	1:300-5000
IP	1-2ug