Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Antibody

$Western blot analysis of HEK293 cell lysate. Using Phospho-RPS6KB1 (Thr421\/Ser424) (bsm-62970R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HEK293 cell lysate. Using Phospho-RPS6KB1 (Thr421\/Ser424) (bsm-62970R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

$25 ug total protein per lane of various lysates (see on figure) probed with Phospho-RPS6KB1 monoclonal antibody, unconjugated (bsm-62970R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Phospho-RPS6KB1 monoclonal antibody, unconjugated (bsm-62970R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Rat

Overview
Catalog #	bsm-62970r
Product Name	Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Antibody
Applications	WB, IP
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human p70 S6 Kinase around the phosphorylation site of T421/S424
Modification Site	Thr421/Ser424
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6198
Swiss Prot	P23443
Synonyms	EC 2.7.11.1, KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, Ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K, kinase p70S6K, p70-S6K.
Background	Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway.
Application Dilution
WB	1:300-5000
IP	1-2ug