MEK5 Recombinant Antibody

$Western blot analysis of Hela cell lysate. Using MEK5 (bsm-63027R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of Hela cell lysate. Using MEK5 (bsm-63027R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-63027r
Product Name	MEK5 Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MEK5
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5607
Swiss Prot	Q13163
Synonyms	MAP2K5; HsT17454; MAPKK 5; MEK 5; MAPKK5; MEK5; MKK5; MAP kinase kinase 5; PRKMK5; MAP kinase kinase MEK5b; MAPK/ERK kinase 5.
Background	Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM.
Application Dilution
WB	1:300-5000
ICC/IF	IFICC1:50-200