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Secondary Antibodies

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Catalog #1: bs-0295G-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 5

Catalog #1: bs-0295G-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 5.5

Catalog #1: bs-0295G-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 7

Catalog #1: bs-0295G-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.

Catalog #1: bs-0295G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0295G-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Peridinin Chlorophyll Protein

Catalog #1: bs-0295G-PE

Applications: WB, FCM

Reactivity: Rabbit

Conjugate: Phycoerythrin

Catalog #1: bs-0295G-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Rhodamine B Isothiocyanate

Catalog #1: bs-0293G

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0293G-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-0293G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0326M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bovine

Conjugate: Unconjugated

Catalog #1: bs-0326M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: Biotin

Catalog #1: bs-0326M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: HRP

Catalog #1: bs-0294M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Goat

Conjugate: Unconjugated

Catalog #1: bs-0294M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: Biotin

Catalog #1: bs-0294M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0370M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Goat

Conjugate: Unconjugated

Catalog #1: bs-0370M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: Biotin

Catalog #1: bs-0370M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0358M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Guinea Pig

Conjugate: Unconjugated

Catalog #1: bs-0358M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: Biotin

Catalog #1: bs-0358M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: HRP

Catalog #1: bs-0297M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0297M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0297M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0297M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0297M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0297M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0345M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0345M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0345M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0295M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0295M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin

Catalog #1: bs-0295M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0369M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0369M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin

Catalog #1: bs-0369M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0293M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0293M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-0293M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0346M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0346M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-0346M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Lane 1: Serum (Mouse) at 30 ug\r\nLane 2: Plasma (Mouse) at 30 ug\r\nLane 3: Liver (Mouse) Tissue Lysate at 40 ug\r\nLane 4: Cerebrum (Mouse) Tissue Lysate at 40 ug\r\nLane 5: Spleen (Mouse) Tissue Lysate at 40 ug\r\nPrimary: Mouse IgA (bs-0774R-HRP) at 1\/1000 dilution\r\nSecondary: Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 55 kD\r\nObserved band size: 62 kD
Lane 1: Serum (Mouse) at 40 ug\r\nLane 2: Small intestine (Mouse) Lysate at 40 ug\r\nLane 3: Lung (Mouse) Lysate at 40 ug\r\nPrimary: Mouse IgA (bs-0774R) at 1\/1000 dilution\r\nSecondary: Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 55 kD\r\nObserved band size: 60 kD

Catalog #1: bs-0774R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0774R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-0774R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-0774R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP