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Secondary Antibodies

Secondary Antibodies

Our Secondary Antibodies support reliable detection in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and related immunoassays. Our collection includes species-specific secondary antibodies against rabbit, mouse, goat, rat, and other primary antibody hosts, with HRP, biotin, fluorophore, and unconjugated formats available to support a wide range of research workflows.



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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.

Catalog #1: bs-0295G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.

Catalog #1: bs-0296G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0293G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0368G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0295D-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0294D-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0293R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0294R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0296R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0403R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0345G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0310G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bs-0295M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0358G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: HRP

Catalog #1: bs-0297G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0336R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Monkey

Conjugate: HRP

Catalog #1: bs-0330R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0432R-HRP

Applications: WB, IHC-P

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bs-0375R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: HRP

Catalog #1: bs-0364R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0303R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Dog

Conjugate: HRP

Catalog #1: bs-0368R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0335R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Monkey

Conjugate: HRP

Catalog #1: bs-0360G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0326R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: HRP

Catalog #1: bs-0297R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0294M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0369M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0327R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: HRP

Catalog #1: bs-0326G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: HRP

Catalog #1: bs-0314R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bsm-0297M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0297M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0293M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0774R-HRP

Applications: WB

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0370R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0345R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0308R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Horse

Conjugate: HRP

Catalog #1: bs-60295g-hrp

Applications: WB

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0377R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0358D-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: HRP

Catalog #1: bs-0346R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0326M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Bovine

Conjugate: HRP

Catalog #1: bs-0310R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bs-0777r-hrp-1-mg

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Other Species

Conjugate: HRP

Catalog #1: bs-0370M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0346M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C\uff0c An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37\u2103\uff0cDAB was used as the chromogen\u3002
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C\uff0c An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37\u2103\uff0cDAB was used as the chromogen

Catalog #1: bs-60296g-hrp-200ul

Applications: IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP