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Secondary Antibodies

Secondary Antibodies

Our Secondary Antibodies support reliable detection in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and related immunoassays. Our collection includes species-specific secondary antibodies against rabbit, mouse, goat, rat, and other primary antibody hosts, with HRP, biotin, fluorophore, and unconjugated formats available to support a wide range of research workflows.



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Catalog #1: bs-0296R-APC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Allophycocyanin

Catalog #1: bs-0296R-BF680

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0296R-BF647

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0296R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cyanine 3

Catalog #1: bs-0296R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cyanine 5

Catalog #1: bs-0296R-BF405

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0296R-PE

Applications: FCM

Reactivity: Mouse

Conjugate: Phycoerythrin

Catalog #1: bs-0296R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-0296R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cyanine 7

Catalog #1: bs-0296R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cyanine 5.5

Catalog #1: bs-0296G-PerCP

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Peridinin Chlorophyll Protein

Catalog #1: bs-0296G-RBITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Rhodamine B Isothiocyanate

Catalog #1: bs-0296G-BF680

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0296G-APC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Allophycocyanin

Catalog #1: bs-0296G-BF750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0296G-PE

Applications: FCM

Reactivity: Mouse

Conjugate: Phycoerythrin

Catalog #1: bs-0296G-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cyanine 7

Catalog #1: bs-0296G-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cyanine 5.5

Catalog #1: bs-0296G-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cyanine 3

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.

Catalog #1: bs-60296g-biotin-200ul

Applications: IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C\uff0c An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37\u2103\uff0cDAB was used as the chromogen\u3002
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C\uff0c An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37\u2103\uff0cDAB was used as the chromogen

Catalog #1: bs-60296g-hrp-200ul

Applications: IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0377R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-0368R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-0330R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-0774R-FITC

Applications: WB

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-0774R-Biotin

Applications: WB

Reactivity: Mouse

Conjugate: Biotin