RECEIVE 25% OFF PUBLISHED ANTIBODIES WITH CODE "PUB25"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Secondary Antibodies

Secondary Antibodies

Our Secondary Antibodies support reliable detection in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and related immunoassays. Our collection includes species-specific secondary antibodies against rabbit, mouse, goat, rat, and other primary antibody hosts, with HRP, biotin, fluorophore, and unconjugated formats available to support a wide range of research workflows.



×

Collection Menu
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.

Catalog #1: bs-0295G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0295G-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0295G

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0295D-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (RAT brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:2000) instructionsand DAB staining.

Catalog #1: bs-0295G-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin

Catalog #1: bs-0295G-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0295G-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: FITC

Catalog #1: bs-0295G-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 5

Catalog #1: bs-0295D

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0295M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0295G-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0295G-PE

Applications: WB, FCM

Reactivity: Rabbit

Conjugate: Phycoerythrin

Catalog #1: bs-0295D-BF488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0295M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0295D-BF647

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0295D-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin

Catalog #1: bs-0295G-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0295D-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: FITC

Catalog #1: bs-0295D-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 7

Catalog #1: bs-0295D-BF594

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0295G-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 405

Catalog #1: bs-60295g

Applications: WB, IHC, IF

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0295D-BF750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0295D-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 5

Catalog #1: bs-0295M-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: FITC

Catalog #1: bs-0295G-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Rhodamine B Isothiocyanate

Catalog #1: bs-0295G-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Allophycocyanin

Catalog #1: bs-0369M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0295D-BF680

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0295D-BF350

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0295D-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 3

Catalog #1: bs-0295M-PerCP

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Peridinin Chlorophyll Protein

Catalog #1: bs-0295M-BF488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0295M-BF750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0295G-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Peridinin Chlorophyll Protein

Catalog #1: bs-0295G-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0295G-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0295G-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 7

Catalog #1: bs-0295G-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 3

Catalog #1: bs-0369M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-60295g-hrp

Applications: WB

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-60295g-bf488

Applications: WB

Reactivity: Rabbit

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0369M-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 5

Catalog #1: bs-0295D-PerCP

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Peridinin Chlorophyll Protein

Catalog #1: bs-0295D-BF405

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0295M-APC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Allophycocyanin

Catalog #1: bs-0295M-PE

Applications: FCM

Reactivity: Rabbit

Conjugate: Phycoerythrin

Catalog #1: bs-0369M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin