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Human U2-OS cells were stained with Osteocalcin Polyclonal Antibody, Unconjugated(bs-4917R) at 1:200 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Human MG63 cell lysates; Lane 2: Human A549 cell lysates probed with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human MG63 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human U-2 OS cell lysates probed with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-4917r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Human Siha cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HepG2 cell lysates probed with CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD14) Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1192R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1\/CD90\/ Thy1.1 Polyclonal Antibody, Unconjugated (bs-0778R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with CD90 Polyclonal Antibody, Unconjugated (bs-0778R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0778R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4834R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat colitis tissue labeled with Anti-PDGFRA Polyclonal Antibody, Unconjugated (bs-0231R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
SH-Sy5y (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDGFRA Antibody (bs-0231R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0231R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-CD105 Polyclonal Antibody (bs-4609R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat brain, L2 rat heart lysates probed (bs-4609R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 70kDa.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Rabbit Anti-CD105 Polyclonal Antibody (bs-4609R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-4609R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4834R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-4834R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-4609R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0778R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0778R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-4609R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-4834R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bs-0231R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-0778R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0231R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-4834R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-4834R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-4834R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bs-0231R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-0231R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-4834R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-4834R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-4834R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0778R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0778R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-4834R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bs-0231R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-0231R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-4609R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-4609R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-4609R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-4609R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-4609R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0778R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0778R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0778R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-4609R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0778R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-0231R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-4834R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-4609R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bs-4609R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bs-4609R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bs-4609R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bs-4609R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bs-4609R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bs-4609R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680