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Unconjugated Primary Antibodies



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Catalog #1: bsm-90388r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90387r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90395r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90380r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90397r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90390r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90393r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90373r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90394r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-90382r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90389r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90392r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90386r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90379r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90378r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90385r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90381r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-90384r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90372r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90375r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90374r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90377r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90376r-50uL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with TFRC monoclonal antibody, unconjugated (bsm-43165M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-TFRC antibody (bsm-41365M): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Mouse IgG (bs-0296P). Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-40296G-FITC): 1 \u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (bsm-43165M) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-43165m-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Recombinant human TLR7 protein, His(bs-41320P) per lane probed with TLR7 polyclonal antibody respectively, unconjugated (bsm-41320R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
The THP-1 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-TLR7 antibody (bsm-41320R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-41320r-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Notch1 monoclonal antibody, unconjugated (bsm-60871R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \r\n
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Notch1 Monoclonal Antibody, Unconjugated(bsm-60871R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Notch1 Monoclonal Antibody, Unconjugated(bsm-60871R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Notch1 Monoclonal Antibody, Unconjugated(bsm-60871R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60871r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Progesterone Receptor) Monoclonal Antibody, Unconjugated (bsm-60804R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Progesterone Receptor Monoclonal Antibody, Unconjugated(bsm-60804R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Progesterone Receptor monoclonal antibody, unconjugated (bsm-60804R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The T47-D (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Progesterone Receptor antibody (bsm-60804R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n

Catalog #1: bsm-60804r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Estrogen receptor alpha) Monoclonal Antibody, Unconjugated (bsm-60803R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human endometrium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Estrogen receptor alpha) Monoclonal Antibody, Unconjugated (bsm-60803R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Estrogen receptor alpha monoclonal antibody, unconjugated (bsm-60803R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Estrogen receptor alpha antibody (bsm-60803R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-60803r-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (bsm-33077M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Lymph); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (bsm-33077M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD5) Monoclonal Antibody, Unconjugated (bsm-33077M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33077m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Normal HeLa cell lysates; Lane 2: HeLa cell treated with Brefeldin A (5 \u03bc g\/ml, 15 min) lysates;Lane 3: Jurkat cell lysates (Negative control ) probed with IL18 Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-IL18 antibody (bsm-56205R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-56205r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD1A ) Monoclonal Antibody, Unconjugated (bsm-60907R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD1A ) Monoclonal Antibody, Unconjugated (bsm-60907R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD1A ) Monoclonal Antibody, Unconjugated (bsm-60907R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD1A ) Monoclonal Antibody, Unconjugated (bsm-60907R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60907r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70513M

Applications: WB, FCM, IP, IHC

Reactivity: Mouse, Rat, Bacteria, Tags

Conjugate: Unconjugated

Catalog #1: bsm-70350M

Applications: WB, FCM, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-70351r

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1534R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-17100R

Applications: ELISA, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-42049R

Applications: ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-20034r

Applications: IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10509R

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43118R

Applications: ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Jurkat cell lysates probed with human CD2 Monoclonal Antibody, Unconjugated (bsm-30002M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
scatter diagram showing PBMC stained with CD2. The cells were incubated with the antibody (bsm-30002M,1\u03bcg\/Test) for 30 min at 22\u00b0C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.

Catalog #1: bsm-30002m-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat nephroblastoma labeled with Anti SET Polyclonal Antibody, Unconjugated (bs-5943R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SET) polyclonal Antibody, Unconjugated (bs-5943R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human HepG2 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A431 cell lysates probed with SET Polyclonal Antibody, Unconjugated (bs-5943R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5943R

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti-ELAVL1\/HUR Polyclonal Antibody, Unconjugated(bs-2651R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti-ELAVL1\/HUR Polyclonal Antibody, Unconjugated (bs-2651R) followed by conjugation to the secondary antibody and DAB staining
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with ELAVL1 Antibody(bs-2651R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-2651R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-OAS1 Polyclonal Antibody, Unconjugated (bs-0687R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with OAS1 Antibody(bs-0687R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0687R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

scatter diagram showing peripheral blood lymphocytes stained with CD25. The cells were incubated with the antibody (bsm-33113M) for 30 min at 22\u00b0C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
SR cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD25 .Antibody(bsm-33113M)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-33113m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Transferrin Receptor (8G6) Monoclonal Antibody, Unconjugated (bsm-52793R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Transferrin Receptor (8G6) Monoclonal Antibody, Unconjugated (bsm-52793R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Transferrin Receptor (8G6) Monoclonal Antibody, Unconjugated (bsm-52793R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Transferrin Receptor (8G6) Monoclonal Antibody, Unconjugated (bsm-52793R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52793r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Spleen tissue lysates ; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Human Jurkat cell lysates; Lane 7: Human Raji cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human Hela cell lysates probed with Peroxiredoxin 1 Monoclonal Antibody, Unconjugated (bsm-33118M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Peroxiredoxin 1) polyclonal Antibody, Unconjugated (bsm-33118M) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Peroxiredoxin 1 Antibody(bsm-33118M)at 1:200 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-33118m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD2) Monoclonal Antibody, Unconjugated (bsm-33075M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD2) Monoclonal Antibody, Unconjugated (bsm-33075M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33075m

Applications: FCM

Reactivity:

Conjugate: Unconjugated