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Unconjugated Primary Antibodies



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Formalin-fixed and paraffin embedded rat tongue labeled with Anti-CK6 Polyclonal Antibody, Unconjugated (bs-1661R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-CK6 Polyclonal Antibody, Unconjugated (bs-1661R) followed by conjugation to the secondary antibody and DAB staining
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Cytokeratin 6) polyclonal Antibody, Unconjugated (bs-1661R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
MCF-7 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Cytokeratin 6 . Antibody(bs-1661R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1661r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-1651r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37\u00b0C for 30min; Antibody incubation with Gli2 Polyclonal Antibody, Unconjugated bs-11564R at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Gli ) Polyclonal Antibody, Unconjugated (bs-11564R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Gli ) Polyclonal Antibody, Unconjugated (bs-11564R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Gli2) polyclonal Antibody, Unconjugated (bs-11564R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-11564r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain (snr) labeled with Anti-5-HTR1B Polyclonal Antibody, Unconjugated (bs-1125R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Placenta tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with 5HT1B Receptor Polyclonal Antibody, Unconjugated (bs-1125R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-1125r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-10305r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human brain glioma labeled with Anti-Activated protein C\/PROC1 Polyclonal Antibody, Unconjugated(bs-0040R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti-Activated protein C\/PROC1 Polyclonal Antibody, Unconjugated(bs-0040R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C
Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Colon tissue lysates; Lane 3: Rat H9C2 cell lysates probed with protein C Polyclonal Antibody, Unconjugated (bs-0040R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-0040r-100ul

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: Unconjugated

100 ng A\u03b21-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-4713M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Positive sample\uff08left\uff09\uff1aAlzheimer's Disease Brain\r\nNegative sample\uff08right\uff09\uff1aNormal Human Brain\r\nBlock endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-4713M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining.

Catalog #1: bsm-4713m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial carcinoma tissue labeled with Anti BACH1\/BRIP1 Polyclonal Antibody,Unconjugated (bs-4092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti BACH1\/BRIP1 Polyclonal Antibody,Unconjugated (bs-4092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BACH1 Polyclonal Antibody, Unconjugated (bs-4092R) at 1:200 overnight at 4\u00b0C, DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BACH1 Polyclonal Antibody(bs-4092R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4092r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-4269r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken

Conjugate: Unconjugated

Catalog #1: bsm-43176m-100ul

Applications: WB

Reactivity: Bovine

Conjugate: Unconjugated

Catalog #1: bs-70558r

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-MAPKAPK2(Ser272)Polyclonal Antibody, Unconjugated (bs-5503R)at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-MAPKAPK2(Ser272)Polyclonal Antibody, Unconjugated (bs-5503R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Phospho-MAPKAPK2 (Ser272) ) Polyclonal Antibody, Unconjugated (bs-5503R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Phospho-MAPKAPK2 (Ser272) ) Polyclonal Antibody, Unconjugated (bs-5503R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-5503r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-OCT1 Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-OCT1 Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (OCT-1 ) Polyclonal Antibody, Unconjugated (bs-1075R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1075r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bsm-70340T

Applications: WB

Reactivity: Human, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70271M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Pig, Rabbit, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-70215M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70143M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70140M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70091M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70126M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70072m-100ul

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70092M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70089M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Drosophila

Conjugate: Unconjugated

Catalog #1: bsm-70038M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70028M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70013M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bsm-70010M

Applications: WB, IHC

Reactivity: Human, Mouse, Bovine, Xenopus

Conjugate: Unconjugated

Catalog #1: bsm-70005M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-70307ch

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-70279ch

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-70222ch

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-70166ch

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-70161r

Applications: WB

Reactivity: Human, Mouse, Bovine

Conjugate: Unconjugated

Catalog #1: bs-70142ch

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-70118r

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Horse

Conjugate: Unconjugated

Catalog #1: bs-70115ch

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Horse

Conjugate: Unconjugated

Catalog #1: bs-70066r

Applications: WB

Reactivity: Human, Rat, Bovine, Dog, Sheep, Rabbit, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bs-70065r

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-70048g

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-70009ch

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-beta-Amyloid 1-40(CT) Polyclonal Antibody, Unconjugated (bs-0877R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U87MG cell lysates; Lane 2: Human SY5Y cell lysates; Lane 3: Human U251 cell lysates probed with beta-Amyloid 1-40 (CT) Polyclonal Antibody, Unconjugated (bs-0877R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0877R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat kidney labeled with Anti-Thrombomodulin\/CD141 Polyclonal Antibody, Unconjugated (bs-0525R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 rat heart, L2 rat brain lysates probed (bs-0525R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 61kDa.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A431 cell lysates ; Lane 5: Human Huvec cell lysates ; Lane 6: Human A549 cell lysates probed with Thrombomodulin Polyclonal Antibody, Unconjugated (bs-0525R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A431 cell lysates ; Lane 5: Human Huvec cell lysates ; Lane 6: Human A549 cell lysates probed with Thrombomodulin Polyclonal Antibody, Unconjugated (bs-0525R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0525R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human cervical carcinoma labeled with Anti-Runx3 Polyclonal Antibody (bs-0378R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse Lymph Node lysates probed with Runx3 Polyclonal Antibody, unconjugated (bs-0378R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0378R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken

Conjugate: Unconjugated

Catalog #1: bs-3128R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-Tau protein(Ser404)Polyclonal Antibody, Unconjugated (bs-2392R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: SH-SY5Y cell lysates; Lane 2: MCF-7 cell lysates probed with Tau protein(Ser579) Polyclonal Antibody, Unconjugated (bs-2392) Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2392R

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-3004R

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-1569R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-MCT1 Polyclonal Antibody, Unconjugated(bs-1569R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-1569R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-71034r

Applications: ELISA/RIA

Reactivity: Bovine

Conjugate: Unconjugated