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Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-iNOS Polyclonal Antibody (bs-0162R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat lung lysates Lane 2: rat brain lysates probed with Anti NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 130kD. Observed band size: 115,130kD.
Lane 1: Mouse Raw264.7 tissue lysates; Lane 2: Human Jurkat cell lysates probed with iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Raw264.7 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a00.1%PBST for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with iNOS Antibody(bs-0162R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0162R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat, Dog, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: HL60 cell lysates probed with IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Rat Lymph node lysates; Lane 3: Rat Thymus lysates probed with IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human K562 cell lysates; Lane 2: Human Jurkat cell lysates probed with IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-1460r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-JNK1\/3 Polyclonal Antibody (bs-0501R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-JNK1 + 3 Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tJNK1 + 3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JNK1+3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0501R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded dog bladder labeled with Anti-Alpha-SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: dog bladder tissue labeled with Anti-Actin A\/Alpha SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300, followed by conjugation to the secondary antibody.
Mouse Small Intestine labeled with Anti-Actin A\/Alpha SMA Polyclonal Antibody, Unconjugated(bs-0189R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C
NIH\/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with alpha Actin Polyclonal Antibody(bs-0189R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0189R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

293T lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A549 lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSK3B (3A6) Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bsm-33293M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN2A\/p16-INK4a Polyclonal Antibody, Unconjugated (bs-20656R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN2A\/p16-INK4a Polyclonal Antibody, Unconjugated (bs-20656R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20656R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with bFGF Polyclonal Antibody, Unconjugated (bs-2235R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with bFGF Polyclonal Antibody, Unconjugated (bs-2235R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with bFGF Polyclonal Antibody, Unconjugated (bs-2235R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with bFGF Polyclonal Antibody, Unconjugated (bs-2235R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2235R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-NNT Polyclonal Antibody, Unconjugated (bs-5097R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NNT Polyclonal Antibody, Unconjugated (bs-5097R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5097R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti DGAT1\/Diglyceride acyltransferase Polyclonal Antibody, Unconjugated (bs-2332R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti DGAT1\/Diglyceride acyltransferase Polyclonal Antibody, Unconjugated (bs-2332R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse intestine lysates probed with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2332R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD34 Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34) Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34) Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34) Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-54470r-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

hepg2 lysates probed with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C. \r\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5551r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4978R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha\/beta II (Thr638\/641) Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-PKC alpha\/beta II (Thr638\/641) Polyclonal Antibody, Unconjugated (bs-3333R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Catalog #1: bs-3333R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded chicken testis tissue labeled with Anti-Neurturin Polyclonal Antibody, Unconjugated(bs-0073R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Frozen mouse brain tissue labeled with Anti-Neurturin Polyclonal Antibody, Unconjugated(bs-0073R) 1:200 followed by conjugation to the secondary antibody
Mouse placenta and heart lysates probed with Rabbit Anti-Neurturin Polyclonal Antibody, Unconjugated (bs-0073R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: Human K562 cell Lysates; Lane 2: Human 293T cell Lysates. Probed with Neurturin polyclonal Antibody, unconjugated (bs-0073R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0073R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat small intestine labeled with Anti-BFGFR\/FGFR1 Polyclonal Antibody, Unconjugated (bs-0230R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FGFR1) Polyclonal Antibody, Unconjugated (bs-0230R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FGFR1) Polyclonal Antibody, Unconjugated (bs-0230R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human meningioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FGFR1) Polyclonal Antibody, Unconjugated (bs-0230R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0230R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken

Conjugate: Unconjugated

Lane 1: mouse lung lysates Lane 2: human colon carcinoma lysates probed with Anti Cryopyrin\/CIAS1\/NALP3 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 114kD. Observed band size: 40kD, 114kD.
RAW264.7 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with NALP3\/CIAS1 Antibody(bs-6655R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-6655R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti FOXO1A Polyclonal Antibody, Unconjugated (bs-9439R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-9439P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FOXO1A Polyclonal Antibody(bs-9439R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Rat Lymph node lysates; Lane 2: Rat Bone lysates; Lane 3: Rat Liver lysates probed with FOXO1A Polyclonal Antibody, Unconjugated (bs-9439R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9439R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-13090R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Unconjugated

Catalog #1: bs-4696r

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with GM130 monoclonal antibody, unconjugated (bsm-61120R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GM130 Monoclonal Antibody, Unconjugated (bsm-61120R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GM130 Monoclonal Antibody, Unconjugated (bsm-61120R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GM130 Monoclonal Antibody, Unconjugated (bsm-61120R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61120r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-IRS1 (Tyr632) ) polyclonal Antibody, Unconjugated (bs-8707R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-8707R

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-4610R

Applications: WB, ELISA

Reactivity: Dog

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Dog testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DNTNP\/FAM53A Polyclonal Antibody, Unconjugated (bs-11839R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DNTNP\/FAM53A Polyclonal Antibody, Unconjugated (bs-11839R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-11839R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Unconjugated

Catalog #1: bs-9459r-100ul

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-TET1 Polyclonal Antibody, Unconjugated (bs-8523R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TET1) polyclonal Antibody, Unconjugated (bs-8523R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.\r\n\r\n
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with TET1 Antibody(bs-8523R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-8523r-100ul

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-P2RX4 Polyclonal Antibody, Unconjugated (bs-7690R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded mouse colon labeled with Anti-P2RX4 Polyclonal Antibody, Unconjugated(bs-7690R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Antigen: bs-7690P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
25 ug total protein per lane of various lysates (see on figure) probed with P2RX4 polyclonal antibody, unconjugated (bs-7690R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-7690r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

hela lysates probed with GPR48 Polyclonal Antibody, Unconjugated (bs-6676R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (GPR48) Polyclonal Antibody, Unconjugated (bs-6676R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-6676r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human A549 cell lysates probed with RAR gamma Polyclonal Antibody, Unconjugated (bs-6218R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (RAR gamma) polyclonal Antibody, Unconjugated (bs-6218R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-6218r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Beta galactosidase Polyclonal Antibody, Unconjugated (bs-4631R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver labeled with Rabbit Anti Beta galactosidase Polyclonal Antibody, Unconjugated (bs-4631R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-4631r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-MYL3\/Myosin light chain 3 Polyclonal Antibody, Unconjugated (bs-2240R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse heart labeled with Anti-MYL3\/Myosin light chain 3 Polyclonal Antibody, Unconjugated (bs-2240R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Muscle lysates probed with MYL3\/Myosin light chain 3 Polyclonal Antibody, Unconjugated (bs-2240R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Muscle tissue lysates; Lane 2: Rat Muscle tissue lysates probed with MYL3 Polyclonal Antibody, Unconjugated (bs-2240R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-2240r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and rat brain tissue labeled with Anti-NCX3 Polyclonal Antibody (bs-2057R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A549 cell lysates probed with NCX3 Polyclonal Antibody, Unconjugated (bs-2057R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-2057r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37\u00b0C for 30min; Antibody incubation with Gli2 Polyclonal Antibody, Unconjugated bs-11564R at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Gli ) Polyclonal Antibody, Unconjugated (bs-11564R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Gli ) Polyclonal Antibody, Unconjugated (bs-11564R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Gli2) polyclonal Antibody, Unconjugated (bs-11564R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-11564r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain (snr) labeled with Anti-5-HTR1B Polyclonal Antibody, Unconjugated (bs-1125R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Placenta tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with 5HT1B Receptor Polyclonal Antibody, Unconjugated (bs-1125R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-1125r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-10305r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human esophageal cancer labeled with Anti-E2F2 Polyclonal Antibody (bs-1001R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: human kidney tissue labeled with Anti-E2F2 Polyclonal Antibody (bs-1001R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1001r-100ul

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: Unconjugated

Catalog #1: bs-44010r-100ul

Applications: WB, ELISA

Reactivity: Dog

Conjugate: Unconjugated

100 ng A\u03b21-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-4713M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Positive sample\uff08left\uff09\uff1aAlzheimer's Disease Brain\r\nNegative sample\uff08right\uff09\uff1aNormal Human Brain\r\nBlock endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-4713M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining.

Catalog #1: bsm-4713m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial carcinoma tissue labeled with Anti BACH1\/BRIP1 Polyclonal Antibody,Unconjugated (bs-4092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti BACH1\/BRIP1 Polyclonal Antibody,Unconjugated (bs-4092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BACH1 Polyclonal Antibody, Unconjugated (bs-4092R) at 1:200 overnight at 4\u00b0C, DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BACH1 Polyclonal Antibody(bs-4092R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4092r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-4269r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken

Conjugate: Unconjugated

Catalog #1: bsm-43168m-100ul

Applications: WB

Reactivity: Dog

Conjugate: Unconjugated

Catalog #1: bsm-43166m-100ul

Applications: WB

Reactivity: Dog

Conjugate: Unconjugated

Catalog #1: bsm-62901r-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated(bsm-61710R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated(bsm-61710R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated(bsm-61710R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated(bsm-61710R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61710r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61391r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Gamma Tubulin Monoclonal Antibody, Unconjugated(bsm-61186R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Gamma Tubulin Monoclonal Antibody, Unconjugated(bsm-61186R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Gamma Tubulin Monoclonal Antibody, Unconjugated(bsm-61186R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Gamma Tubulin Monoclonal Antibody, Unconjugated(bsm-61186R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61186r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70085M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-70066r

Applications: WB

Reactivity: Human, Rat, Bovine, Dog, Sheep, Rabbit, Guinea Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-beta-Amyloid 1-40(CT) Polyclonal Antibody, Unconjugated (bs-0877R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U87MG cell lysates; Lane 2: Human SY5Y cell lysates; Lane 3: Human U251 cell lysates probed with beta-Amyloid 1-40 (CT) Polyclonal Antibody, Unconjugated (bs-0877R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0877R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated