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Unconjugated Primary Antibodies



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Catalog #1: bsm-62172r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62153r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with IDH1 monoclonal antibody, unconjugated (bsm-62156R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IDH1 Monoclonal Antibody, Unconjugated(bsm-62156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62156r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-NAP1L1 antibody (bsm-62161R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NAP1L1) monoclonal Antibody, unconjugated (bsm-62161R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with NAP1L1 monoclonal antibody, unconjugated (bsm-62161R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NAP1L1 Monoclonal Antibody, Unconjugated (bsm-62161R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62161r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62154r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62164r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62168r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62177r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62150r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62162r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62132r

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62145r

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62148r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62146r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62144r

Applications: WB, IP, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62133r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62149r

Applications: WB, IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62140r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62137r

Applications: WB, IP, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62141r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62116r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62131r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62139r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62129r

Applications: WB, IP, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62125r

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62121r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62111r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62126r

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62120r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62110r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62115r

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62117r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62112r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62102r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62106r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62114r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62108r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62105r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62101r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62095r

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62096r

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62086r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62081r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62083r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62089r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62082r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62078r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62079r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated