NEK7 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with NEK7 monoclonal antibody, unconjugated (bsm-62095R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with NEK7 monoclonal antibody, unconjugated (bsm-62095R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62095r-100ul
Product Name	NEK7 Recombinant Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NEK7
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	140609
Swiss Prot	Q8TDX7
Subcellular location	Cytoplasm, Nucleus
Synonyms	NEK7; NimA related protein kinase 7.
Background	Protein kinase which plays an important role in mitotic cell cycle progression.Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100