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Unconjugated Primary Antibodies



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Catalog #1: bs-2003R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-1420R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-13383R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-11178R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0763R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-10066R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-0697R

Applications: ELISA, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-10030R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-4696r-100ul

Applications: ELISA

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-0239r-100ul

Applications: ELISA

Reactivity: Virus, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs-6313P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 4HNE-BSA (0.1 \u00b5g); Lane 2: BSA (0.1 \u00b5g) probed with 4 Hydroxynonenal Polyclonal Antibody, Unconjugated (bs-6313R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20159R

Applications: WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (tri methyl K4) Monoclonal Antibody, Unconjugated (bsm-33110M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3 (tri methyl K4)) Monoclonal Antibody, Unconjugated (bsm-33110M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33110m

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-18214r

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-44023m

Applications: WB

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-70146M

Applications: WB, IHC

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bsm-70054M

Applications: WB, IHC

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-70359r

Applications: WB

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-51118r-50ug

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-51093r-50ug

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-51008r-50ug

Applications: WB

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-51122r-50ug

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Rat, Others, Zebrafish, Drosophila

Conjugate: Unconjugated

Catalog #1: bs-51114r-50ug

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-51103R

Applications: WB, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-51015R

Applications: WB, ELISA, IF

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-51014R

Applications: WB, ELISA, IF

Reactivity: Human, Others

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Monkey brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Zebrafish brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U251 cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51566m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-33046M

Applications: ELISA

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-20021R

Applications: WB, ELISA

Reactivity: PCV2

Conjugate: Unconjugated

Antigen: bs-5512P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in Microplate Reader by 450nm\\n
Raji cell lysates probed with NFKB1 (Ser337) Polyclonal Antibody, Unconjugated (bs-5512R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nNFKB1 (Ser337) Polyclonal Antibody(bs-5512R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB1 (Ser337) Polyclonal Antibody, Unconjugated (bs-5512R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5512R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Antigen: bs-5390P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in Microplate Reader by 450nm\\n
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nSeladin 1 Polyclonal Antibody, Unconjugated (bs-5390R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5390R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3417R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33005m

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33119M

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33018m

Applications: WB, ELISA

Reactivity: Human, Pig, Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33183M

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33181M

Applications: WB

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-33184M

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-49135m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-52888R

Applications: WB, IHC-P

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-52887R

Applications: WB

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bsm-52960r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-54203R

Applications: ELISA

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-52350R

Applications: WB

Reactivity: Others

Conjugate: Unconjugated

ChIP assays were performed using HeLa cells, the Bioss antibody against H3K27me1 (Cat. No. bs-53108R) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration of the antibody consisting of 1, 2, 5 and 10 \u03bcg per ChIP experiment was analyzed. IgG (2 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the active gene GAPDH used as a negative and a positive control target, respectively. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H3K27me1 (Cat. No. bs-53108R). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:32,900.
A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H3K27me1 (Cat. No. bs-53108R) with peptides containing other modifications and unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.
Histone extracts (15 \u03bcg) from HeLa cells were analyzed by Western blot using the Bioss antibody against H3K27me1 (Cat. No. bs-53108R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Catalog #1: bs-53108R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Others

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analyzed by Western blot using the Bioss antibody against H3K9me1 (Cat. No. bs-53120R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H3K9me1 (bs-53120R) with peptides containing other modifications and the unmodified sequence of histone H3. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows specificity of the antibody for the modification of interest.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against human H3K9me1 (Cat. No. bs-53120R), crude serum and flow through in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:68,000.
ChIP assays were performed using human HeLa cells, the Bioss antibody directed against H3K9me1 (Cat. No. bs-53120R) and optimized PCR primer sets for qPCR. ChIP was performed with a Low Cell# ChIP kit, using sheared chromatin from 10,000 cells. 1 and 5 \u03bcg of the antibody and 5 \u03bcg of IgG (negative IP control) were used per ChIP experiment. qPCR was performed with primers for the GAPDH promoter and for the inactive gene MYOD. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K9me1 is preferably present at silent genes.

Catalog #1: bs-53120R

Applications: WB, ELISA, dot-blot

Reactivity: Human, Others

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analyzed by Western blot using the Bioss antibody against H3K9me2 (Cat. No. bs-53118R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H3K9me2 (Cat. No. bs-53118R) with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.
Mouse NIH3T3 cells were stained with the Bioss antibody against H3K9me2 (Cat. No. bs-53118R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody against H3K9me2 (Cat. No. bs-53118R), crude serum and Flow through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.

Catalog #1: bs-53118R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H3K36me3 (bs-53116R) with peptides containing other H3 and H4 modifications and the unmodified sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H3K36me3 (Cat. No. bs-53116R) and the crude serum. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:19,300.
ChIP was performed with 2 \u03bcg of the Bioss antibody against H3K36me3 (bs-53116R) on sheared chromatin from 1 million HeLaS3 cells using a ChIP-seq kit. IgG (2 \u03bcg\/IP) was used as a negative IP control. The IP\u2019d DNA was analyzed by qPCR with optimized PCR primer pairs for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the coding region of the active ACTB gene (figure A). The IP\u2019d DNA was subsequently analyzed on an Illumina Genome Analyzer. Library preparation, cluster generation, and sequencing were performed according to the manufacturer\u2019s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure B shows the obtained profiles in genomic regions of chromosome 12 (including the GAPDH positive control), 7 (including the ACTB positive control), 14 and 3, respectively. These results clearly show an enrichment of the H3K36me3 at active genes.
ChIP assays were performed using HeLa cells, the Bioss antibody against H3K36me3 (Cat. No. bs-53116R) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells on the SX-8G IP-Star automated system. A titration consisting of 1, 2, 5 and 10 \u03bcg of antibody per ChIP experiment was analyzed. IgG (2 \u03bcg\/IP) was used as a negative IP control. QPCR was performed with primers for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the Sat2 satellite repeat. The figure shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53116R

Applications: WB, ELISA, dot-blot, ChIP-seq

Reactivity: Human, Others, Zebrafish, Drosophila

Conjugate: Unconjugated

A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H4K20me3 (Cat. No. bs-53115R) with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest
Human osteosarcoma (U2OS) cells were stained with the Bioss antibody against H4K20me3 (Cat. No. bs-53115R) and with DAPI. Cells were fixed with ice-cold methanol for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H4K20me3 antibody (left) diluted 1:300 in blocking solution followed by an anti-rabbit secondary antibody conjugated to Alexa568 or with DAPI (right). Figure B: staining of the cells with the H4K20me3 antibody after incubation of the antibody with blocking peptide at 5 ng\/\u03bcl.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H4K20me3 (Cat. No. bs-53115R), crude serum and flow through in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:7,400.
ChIP assays were performed using HeLa cells, the Bioss antibody against H4K20me3 (Cat. No. bs-53115R) and optimized PCR primer sets for qPCR. ChIP was performed with an Auto Histone ChIP-seq kit with sheared chromatin from 1 million cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 \u03bcg per ChIP experiment was analyzed. IgG (1 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for promoters of the active genes c-fos and GAPDH, used as negative controls, and for the Sat2 satellite repeat region used as a positive control. The figure shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53115R

Applications: WB, ELISA, IHC-P, IF(ICC), IF, dot-blot, ChIP-seq

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated