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Unconjugated Primary Antibodies



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Catalog #1: bsm-41621M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-60258m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60521m

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60317m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30098M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: HepG2, Neuro-2a, EL4.IL-2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 S\r\nPredicted MW: 86 kDa\r\nObserved MW: 86 kDa
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HepG2 cell lysates probed with Mitofusin 2 Monoclonal Antibody, Unconjugated (bsm-60007M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60007M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-41489m-100ul

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-41488m-100ul

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Monkey brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Zebrafish brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U251 cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51566m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-51648m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51564m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: MCF-7 cell lysates probed with Nm23\/NME1 Monoclonal Antibody, Unconjugated (bsm-51560M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51560m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51534m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52008m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51099M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: 293; Lane 2: Hela whole cell lysate at 20 \u00b5g per lane; probed with bsm-51055M PRKAA2 (206CT5.4.2) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), incubated with PRKAA2 (206CT5.4.2) Monoclonal Antibody (bsm-51055M) at 1:25 dilution for 1 h at 37\u2103, followed by secondary antibody incubation (green), actin (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51055M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51414M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Sample Tissue\/Cells lysates probed with antibodyname Monoclonal Antibody, unconjugated (bsm-51413M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.
Paraformaldehyde-fixed, paraffin embedded Human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NME1 (1172CT2.4.1.1) Monoclonal Antibody (bsm-51413M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51413M

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with YY1 (3C6) Monoclonal Antibody (bsm-51412M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U-251 cell lysates; probed with YY1 (3C6) Polyclonal Antibody, unconjugated (bsm-51412M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Monkey brain tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with YY1 (3C6) Monoclonal Antibody, Unconjugated (bsm-51412M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51412M

Applications: WB, FCM, IHC-P

Reactivity: Human, Rat, Monkey

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: K562 Cell lysates; Lane 3: Daudi Cell lysates; probed with PLIN3 (1C4) Monoclonal Antibody, unconjugated (bsm-51361M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HUVEC cells probed with using PLIN3 (1C4) Monoclonal Antibody (green) unconjugated bsm-51361M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells; Antibody incubation with PLIN3 (1C4) Monoclonal Antibody, Unconjugated (bsm-51361M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). The nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51361M

Applications: WB, FCM, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Raji Cell lysates; probed with RASSF2 (9C9) Monoclonal Antibody, unconjugated (bsm-51348M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using RASSF2 (9C9) Monoclonal Antibody bsm-51348M at 1:25 for 30 minutes followed by A488 secondary antibody at 1:200 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51348M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51347M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51315M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HeLa Cell lysates; Lane 3: K562 Cell lysates; Lane 4: MCF-7 Cell lysates; Lane 5: NIH\/3T3 Cell lysates; probed with CDK4 (9C3) Monoclonal Antibody, unconjugated (bsm-51314M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDK4 (9C3) Monoclonal Antibody (bsm-51314M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining

Catalog #1: bsm-51314M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

ICC analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with Zap70 (4C2) Monoclonal Antibody (bsm-51298M) at 1:25 dilution, followed by secondary antibody incubation. Immunofluorescence image shows cytoplasm staining of Zap70 (green), actin (red), and nuclear counter stain with DAPI (blue).
Lane 1: Jurkat Cell lysates; Lane 2: MOLT-4 Cell lysates; Lane 3: mouse thymus Cell lysates; probed with Zap70 (4C2) Monoclonal Antibody, unconjugated (bsm-51298M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Jurkat cells probed with using Zap70 (4C2) Monoclonal Antibody, bsm-51298M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51298M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51240M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse liver cell lysates; probed with CAT (626CT4.3.8) Monoclonal Antibody, unconjugated (bsm-51185M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cell lysates; probed with CAT (626CT4.3.8) Monoclonal Antibody, unconjugated (bsm-51185M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51185M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated