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Catalog #1: bs-3114R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRS1(Ser1101) Polyclonal Antibody, Unconjugated (bs-3197R) at 1:400 overnight at 4\u00b0C, DAB staining.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS1(Ser1101) Polyclonal Antibody(bs-3197R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRS1(Ser1101) Polyclonal Antibody, Unconjugated (bs-3197R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRS1(Ser1101) Polyclonal Antibody, Unconjugated (bs-3197R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3197R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti Phospho-Bcl-2(Ser70) Polyclonal Antibody, Unconjugated (bs-3052R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with Anti-Bcl-2 (Ser70) Polyclonal Antibody, Unconjugated (bs-3052R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Image submitted by OWL Validation Program. 20 \u03bcg proteins of cell lysates from A431, U266 and THP-1 were separated by SDS-PAGE and transferred to PVDF membrane for western blotting analysis antibodies against bs-3052r Rabbit Anti-Bcl-2 (Ser70) Polyclonal Antibody at dilution of 1:1000 with exposure time of 10 min.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl-2 (Ser70) Polyclonal Antibody, Unconjugated (bs-3052R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3052R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Phospho-FRA1 (Ser265)Polyclonal Antibody, Unconjugated (bs-3158R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Hela cell lysates; Lane 2: U87MG cell lysates probed with Phospho-FRA1 (Ser265) Polyclonal Antibody, Unconjugated (bs-3158R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3158R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse embryo lysates probed with Anti Phospho-CDK9 (Thr186) Polyclonal Antibody, Unconjugated (bs-3097R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 43kD. Observed band size: 43kD.
Lane 1: Mouse Brain lysates probed with CDK9 (Thr186) Polyclonal Antibody, Unconjugated (bs-3097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3097R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-phospho-IRS-1(Ser307) Polyclonal Antibody, Unconjugated (bs-2736R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS-1(Ser307) Polyclonal Antibody(bs-2736R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2736R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Molt4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-NFATc2 (Ser326) Antibody(bs-2669R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
U251 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-NFATc2 (Ser326) Polyclonal Antibody(bs-2669R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-2669R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: mouse heart lysates Lane 2: mouse S\/P20 cell lysates probed with Anti phospho-ERK1(Thr202\/Tyr204)+ERK2(Thr183\/Tyr185) Polyclonal Antibody, Unconjugated (bs-1646R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size:42kD
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-ERK1(Thr202\/Tyr204)+ERK2(Thr183\/Tyr185) Polyclonal Antibody, Unconjugated (bs-1646R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1(T202\/Y204)+ERK2(T183\/Y185) Polyclonal Antibody, Unconjugated (bs-1646R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1(T202\/Y204)+ERK2(T183\/Y185) Polyclonal Antibody, Unconjugated (bs-1646R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1646R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Raji cell lysates probed with phospho-Fos B (Ser27) Polyclonal Antibody, Unconjugated (bs-13198R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Siha cell lysates probed with phospho-Fos B (Ser27) Polyclonal Antibody, Unconjugated (bs-13198R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-13198R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13384R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Cerebral cortex lysates probed with Nemo-like kinase (Thr298) Antibody, Unconjugated (bs-10421R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nemo-like kinase (Thr298) Polyclonal Antibody, Unconjugated (bs-10421R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-10421R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse lung cells probed with Rabbit Anti-phospho-Ron (Tyr1238+Tyr1239) Polyclonal Antibody, FITC Conjugated (bs-10118R-FITC) at 1:100 for 40 minutes.
Lovo cell lysates probed with phospho-Ron (Tyr1238+Tyr1239) Polyclonal Antibody, Unconjugated (bs-7537R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10118R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Polyclonal Antibody, Unconjugated (bs-8813R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebral cortex lysates probed with AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Polyclonal Antibody, Unconjugated (bs-8813R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8813R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0636R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A431 cell lysates probed with phospho-CDK7 (Thr170) Polyclonal Antibody, Unconjugated (bs-10997R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-CDK7 (Thr170)) polyclonal Antibody, Unconjugated (bs-10997R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-CDK7 (Thr170)) polyclonal Antibody, Unconjugated (bs-10997R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-10997R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52205r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PKR(T446) (10A1) Monoclonal Antibody, Unconjugated (bsm-52189R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PKR(T446) (10A1) Monoclonal Antibody, Unconjugated (bsm-52189R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Hcclm3 cell lysates probed with PKR(T446) (10A1) Monoclonal Antibody, Unconjugated (bsm-52189R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKR (Thr446) Monoclonal Antibody, Unconjugated (bsm-52189R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52189r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H2A.X(S139) (1C14) Monoclonal Antibody, Unconjugated (bsm-52163R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52163r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A549; Lane 2: HCT116; Lane 3: Hela; Lane 4: HepG2 cell lysates; probed with c-Myc(S62) (1A7 ) Monoclonal Antibody (bsm-52143R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HeLa cells were stained with c-Myc(S62) (1A7 ) Monoclonal Antibody (bbsm-52143R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with c-Myc(S62) (1A7) Monoclonal Antibody, Unconjugated (bsm-52143R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with c-Myc(S62) (1A7) Monoclonal Antibody, Unconjugated (bsm-52143R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52143r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6728r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-5577R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-5477R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Phospho-FGFR1+FGFR2 (Tyr463) Polyclonal Antibody, Unconjugated (bs-5326R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with FGFR1\/FGFR2 (Tyr463\/Tyr466) Antibody (bs-5326R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
U87MG cell lysates probed with Phospho-FGFR1+FGFR2 (Tyr463\/Tyr466) Polyclonal Antibody, Unconjugated (bs-5326R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463\/Tyr466)) Polyclonal Antibody, Unconjugated (bs-5326R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-5326R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Dnmt1(Ser154) Polyclonal Antibody(bs-5294R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dnmt1(Ser154) Polyclonal Antibody, Unconjugated (bs-5294R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dnmt1(Ser154) Polyclonal Antibody, Unconjugated (bs-5294R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dnmt1(Ser154) Polyclonal Antibody, Unconjugated (bs-5294R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5294R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3398r

Applications: WB, ELISA, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti- phospho-PRKC Polyclonal Antibody, Unconjugated (bs-2505R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI
Molt-4 cells were fixed with 4% PFA for 10min at room temperature\uff0cpermeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PKC beta 1\/2 (Thr500) Antibody at 1\uff1a50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed bysecondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.

Catalog #1: bs-2505R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela cell lysates probed with PAK4\/5(Ser99) Polyclonal Antibody, Unconjugated (bs-2270R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-PAK4 (Ser99) Antibody(bs-2270R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-2270R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-1657R

Applications: ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-12447R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-11993R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-8853R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NeuroD1 (Ser274) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NeuroD1 (Ser274) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin-embedded Human Brain Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NeuroD1 (Ser274) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4\u00b0C, followed by secondary antibody, DAB and counterstaining.
Lane 1: Rat Cerebrum lysates; Lane 2: Mouse Cerebellum lysates probed with phospho-NeuroD1 Polyclonal Antibody, Unconjugated (bs-19218R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-19218R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9582R

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad3 (Ser213) Polyclonal Antibody(bs-5459R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad3 (Ser213) Polyclonal Antibody, Unconjugated (bs-5459R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad3 (Ser213) Polyclonal Antibody, Unconjugated (bs-5459R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad3 (Ser213) Polyclonal Antibody, Unconjugated (bs-5459R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human myometrium labeled with Anti phospho-ERK5(Ser731+Thr733)Polyclonal Antibody, Unconjugated (bs-5486R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human lung cancer labeled with Rabbit Anti-ERK5 (Ser731+Thr733) Polyclonal Antibody, Unconjugated(bs-5486R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK5 ( Ser731+Thr733) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Rat Muscle tissue lysates; Lane 5: Rat Cerebrum tissue lysates probed with phospho-ERK5 ( Ser731+Thr733) Polyclonal Antibody, Unconjugated (bs-5486R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5486R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A431 cell lysates probed with FAK (Tyr925) Polyclonal Antibody, Unconjugated (bs-5540R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FAK(Tyr925) Polyclonal Antibody, Unconjugated (bs-5540R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FAK(Tyr925) Polyclonal Antibody, Unconjugated (bs-5540R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FAK(Tyr925) Polyclonal Antibody, Unconjugated (bs-5540R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5540R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse bone lysates probed with RAD51(Thr309) Polyclonal Antibody, Unconjugated (bs-5677R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Bone lysates probed with RAD51(Thr309) Polyclonal Antibody, Unconjugated (bs-5677R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RAD51(Thr309) Polyclonal Antibody, Unconjugated (bs-5677R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RAD51(Thr309) Polyclonal Antibody, Unconjugated (bs-5677R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5677R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-5592R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Lane 1: Mouse Cerebellum tissue lysates; Lane 2: Rat Cerebellum tissue lysates probed with phospho-Gria2 (Ser880) Polyclonal Antibody, Unconjugated (bs-5359R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Gria2 (Ser880) ) Polyclonal Antibody, Unconjugated (bs-5359R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-5359R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti phospho-AKT1\/2\/3 (Tyr315\/316\/312) Polyclonal Antibody, Unconjugated (bs-5193R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.
Lane 1: Mouse Adrenal glands lysates; Lane 2: Mouse Cerebrum lysates probed with phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) Polyclonal Antibody, Unconjugated (bs-5193R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) ) polyclonal Antibody, Unconjugated (bs-5193R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-5193R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4004R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse muscle lysates probed with TAK1(Thr187) Polyclonal Antibody, Unconjugated (bs-3438R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human K562 cell lysates probed with TAK1 Polyclonal Antibody, Unconjugated (bs-3438R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TAK1(Thr187) Polyclonal Antibody, Unconjugated (bs-3438R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3438R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-3430P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Stat4 (Tyr693) Polyclonal Antibody, Unconjugated (bs-3430R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3430R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Phospho-Met(Tyr1234\/1235)Polyclonal Antibody, Unconjugated (bs-3272R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image was kindly submitted by An anonymous end user. Human cell line stained with RRabbit Anti-Phospho-Met(Tyr1234 + Tyr1235) Polyclonal Antibody (bs-3272R).

Catalog #1: bs-3272R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3371R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3238R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-Phospho-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1:A431 lysates Lane 2: Mouse stomach probed with Rabbit Anti-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse muscle lysates probed with Rabbit Anti-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with Phospho-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3395R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-3091P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-CDK1 (Thr14)) polyclonal Antibody, Unconjugated (bs-3091R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-CDK1 (Thr14) Polyclonal Antibody(bs-3091R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Mouse Thymus tissue lysates; Lane 4: Rat Thymus tissue lysates ; Lane 5: Human SH-SY5Y cell lysates probed with Phospho-CDK1 (Thr14) Polyclonal Antibody, Unconjugated (bs-3091R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3091R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated