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Unconjugated Primary Antibodies



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Catalog #1: bs-2616R

Applications: WB, ELISA, FCM, IP

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat exfoliated cells labeled with Anti-CXCR3 Polyclonal Antibody, Unconjugated (bs-2209R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HCCLM3 cells probed with Rabbit Anti-CXCR3 Polyclonal Antibody, Unconjugated (bs-2209R ) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Blood cell tissue lysates; Lane 2: Rat Blood cell tissue lysates probed with CXCR3 Polyclonal Antibody, Unconjugated (bs-2209R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2209R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Annexin A13 Polyclonal Antibody, Unconjugated (bs-12478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lymphoma labeled with Rabbit Anti alpha MSH Polyclonal Antibody, Unconjugated (bs-1848R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse foot tumor labeled with Anti-\u03b1-MSH, Unconjugated (bs-1848R) followed by conjugation to the secondary antibody
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with alpha MSH Polyclonal Antibody, Unconjugated (bs-1848R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bs-1848R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) used at 1:200 dilution for 40 minutes at 37\u00b0C

Catalog #1: bs-2934R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
U-87MG cells were fixed with 4% PFA for 10min at room temperature \uff0cpermeabilized with 20% PBST for 20 min at room temperature , and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PERK Polyclonal Antibody at 1\uff1a50 dilution in blocking buffer and incubated for 30 min at -20\u2103, washed twice with 2%BSA in PBS, followed bysecondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.
Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2469R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Image kindly submitted by Jennifer DeMuro as part of the Free Sample Program. Mouse brain lysates probed with Rabbit Anti-Leptin receptor Polyclonal Antibody (bs-0961R).
K562 cells probed with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 3ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Leptin receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Liver; Probed with Leptin Receptor Polyclonal Antibody, Unconjugated (bs-0961R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0961R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti AXL\/UFO Polyclonal Antibody, Unconjugated (bs-5180R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti AXL\/UFO Polyclonal Antibody, Unconjugated (bs-5180R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human MKN45 cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human FHC cell lysates probed with AXL Polyclonal Antibody, Unconjugated (bs-5180R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Testis lysates; Lane 2:Human Hela cell lysates probed with AXL Polyclonal Antibody, Unconjugated (bs-5180R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5180R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti-oxLDL Polyclonal Antibody, Unconjugated (bs-1698R) followed by conjugation to the secondary antibody and DAB staining
Hcclm3 lysates probed with ox-LDL Polyclonal Antibody, Unconjugated (bs-1698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ox-LDL Polyclonal Antibody, Unconjugated (bs-1698R) at 1:400 overnight at 4\u00b0C, DAB staining.
A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nox-LDL Antibody\uff08bs-1698R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Catalog #1: bs-1698R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebral cortex; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NeuN Polyclonal Antibody, Unconjugated bs-1613R at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1613R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tibia tissue labeled with Anti-Osteoprotegerin Polyclonal Antibody (bs-0431R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-OPG Polyclonal Antibody, Unconjugated (bs-0431R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse heart lysates; Lane 2: Mouse kidney lysates; Lane 3: Mouse liver lysates probed with OPG Polyclonal Antibody (bs-0431R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Lane 1: Human Raji cell lysates; Lane 2: Human U251 cell lysates probed with OPG Polyclonal Antibody, Unconjugated (bs-0431R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0431R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0014R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rabbit pancreas labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-4563R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-4563R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-MMP9 Polyclonal Antibody, Unconjugated (bs-4593R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 mouse brain lysates L2 mouse heart lysates probed with Anti MMP9 Polyclonal Antibody, Unconjugated (bs-4593R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 78kD. Observed band size: 78kD.

Catalog #1: bs-4593R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Rabbit Anti-TEM1\/CD248 Polyclonal Antibody (bs-2101R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Rabbit Anti-TEM1\/CD248 Polyclonal Antibody (bs-2101R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Human 293T cells probed with Rabbit Anti-TEM1\/CD248 Polyclonal Antibody, Unconjugated (bs-2101R) (green) at 1:50 for 40 minutes followed by a conjugated secondary compared to control cells (blue).
Mouse spleen lysates probed with TEM1 Polyclonal Antibody, unconjugated (bs-2101R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-2101R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-Sclerostin Polyclonal Antibody, Unconjugated(bs-10200R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Sclerostin) Polyclonal Antibody, Unconjugated (bs-10200R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Sclerostin) Polyclonal Antibody, Unconjugated (bs-10200R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-10200R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

RSC96 cells probed with CD47\/MER6 Polyclonal Antibody, Unconjugated (bs-2386R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Bone lysates probed with CD47\/MER6 Polyclonal Antibody, Unconjugated (bs-2386R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart lysates; Lane 2: Mouse Kidney lysates probed with CD47\/MER6 Polyclonal Antibody, Unconjugated (bs-2386R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CD47) polyclonal Antibody, Unconjugated (bs-2386R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2386r

Applications: WB, ELISA, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2016R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ATF6 Polyclonal Antibody, Unconjugated (bs-1634R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U251 cells were stained with ATF6 Polyclonal Antibody (bs-1634R) at 1:200 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Pancreas lysates probed with ATF6 Polyclonal Antibody, Unconjugated (bs-1634R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Huvec cell lysates probed with ATF6 Polyclonal Antibody, Unconjugated (bs-1634R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1634R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human tumor tissue labeled with Anti-HPV16-E6 Polyclonal Antibody, Unconjugated(bs-0990R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HPV16 E6 protein Polyclonal Antibody, Unconjugated (bs-0990R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with XXX Antibody(bs-R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange). Antibody(bs-0990R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0990R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Virus

Conjugate: Unconjugated

Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates probed with Collagen 3 Polyclonal Antibody, Unconjugated (bs-0549R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates probed with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-0549r-TR

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Lovo cell lysates; Lane 2: A549 cell lysates probed with HHLA2 Polyclonal Antibody, Unconjugated (bs-8419R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (HHLA2) Polyclonal Antibody, Unconjugated (bs-8419R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-8419R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon cancer labeled with Anti Amphiregulin Polyclonal Antibody, Unconjugated (bs-3847R) at 1:600 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded mouse fetal liver labeled with Rabbit Anti-Amphiregulin Polyclonal Antibody, Unconjugated(bs-3847R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Catalog #1: bs-3847R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Megalin Polyclonal Antibody, Unconjugated (bs-3909R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Megalin Polyclonal Antibody(bs-3909R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3909R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse placenta lysates probed with Rabbit Anti-Transferrin receptor Polyclonal Antibody, Unconjugated (bs-0988R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Transferrin receptor Polyclonal Antibody (bs-0988R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Raji cells probed with \\tTransferrin receptor Polyclonal Antibody, Unconjugated (bs-0988R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Transferrin receptor Polyclonal Antibody(bs-0988R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0988R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0163R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-RNF43 Polyclonal Antibody, Unconjugated(bs-7007R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse testis labeled with Anti RNF43 Polyclonal Antibody, Unconjugated (bs-7007R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Kidney lysates; Lane 3: Mouse Heart lysates; Lane 4: Rat Cerebrum lysates ; Lane 5: Rat Kidney lysates probed with RNF43 Polyclonal Antibody, Unconjugated (bs-7007R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HL-60 cell lysates probed with RNF43 Polyclonal Antibody, Unconjugated (bs-7007R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7007R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Raw264.7 cell lysates probed with IL17RA\/CD217 Polyclonal Antibody, Unconjugated (bs-2606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Raw264.7 cell lysates probed with \r\nIL17RA\/CD217 Polyclonal Antibody, Unconjugated (bs-2606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2606R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse small intestine labeled Anti-GDF8\/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse skeletal muscle lysate probed with Anti GDF8\/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 43kD. Observed band size: 43kD
Generously provided by Bradley Elliott from University of Westminster as part of the Bioss Discovery Program. Lanes 1 and 2 are human muscle lysates and Lanes 3 and 4 are C2C12 cells. Rabbit Anti-GDF8\/MSTN Polyclonal Antibody (bs-1288R) was used at a dilution of 1:1000, overnight at 4C.
Lane 1: Mouse Muscle lysates; Lane 2: Rat Muscle ysates; Lane 3: Rat Tongue lysates probed with GDF8\/MSTN Polyclonal Antibody, Unconjugated (bs-1288R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1288R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2514R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse heart tissue labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution for 40 minutes at 37\u00b0C
Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

Catalog #1: bs-0646r-TR

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6763R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti CCR7\/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CCR7\/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Rabbit Anti-CCR7\/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Raji probed with CCR7\/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1305R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse brain lysates Lane 2: mouse kidney lysates probed with Anti Smad3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 47kD. Observed band size: 47kD.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Ovary lysates; Lane 3: HT1080 cell lysates; Lane 4: Jurkat cell lysates probed with Smad2\/3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad2\/3 Polyclonal Antibody(bs-3484R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Testis lysates; Lane 4: Mouse Skin lysates; Lane 5: Mouse Kidney lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Testis lysates; Lane 8: Rat Kidney lysates; Lane 9: Human HUVEC cell lysates; Lane 10: Human A549 cell lysates; Lane 11: Human Hela cell lysates; Lane 12: Human HT1080 cell lysates; Lane 13: Human A431 cell lysates probed with Smad2\/3 Polyclonal Antibody, Unconjugated (bs-3484R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3484R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Rat kidney lysate at 40\u00b5g; Probed with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:1000 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.
Mouse kidney lysate at 40\u00b5g; Probed with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:1000 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-2957r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1665r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human MG-63 lysates probed with Rabbit Anti-P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
MCF-7 cells were stained with P21 Polyclonal Antibody, Unconjugated(bs-10129R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Paraformaldehyde-fixed, paraffin-embedded Human Hepatoma tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with P21 Polyclonal Antibody, Unconjugated (bs-10129R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bs-10129R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse ovary lysates L2 mouse brain lysates probed with Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 32kD. Observed band size:32kD.\\n
Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-3570R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-LAMP2\/CD107B Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2379R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat exfoliated cells tissue labeled with Anti-CCR4 Polyclonal Antibody, Unconjugated (bs-1168R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat cells labeled with Anti-CCR4\/CD194 Polyclonal Antibody, Unconjugated (bs-1168R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human hepatitides tissue labeled with Anti-CCR4 Polyclonal Antibody, Unconjugated (bs-1168R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1168R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human 293T cells probed with DRD1 Polyclonal Antibody, unconjugated (bs-1007R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Mouse cerebrum lysates probed with DRD1 Polyclonal Antibody, unconjugated (bs-1007R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Mouse olfactory bulb lysates probed with DRD1 Polyclonal Antibody, Unconjugated (bs-1007R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with DRD1 Polyclonal Antibody, Unconjugated (bs-1007R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1007R

Applications: IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat aorta labeled with Anti-LOX-1 Polyclonal Antibody, Unconjugated (bs-2044R) followed by conjugation to the secondary antibody and DAB staining
Human A549 cells probed with Rabbit Anti-LOX1 Polyclonal Antibody, Unconjugated (bs-2044R) (green) at 1:20 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
This image was generously provided by Zu Yue Deng from Zhejiang University of Technology. Human heart tissue probed with Rabbit Anti-LOX 1 Polyclonal Antibody, Unconjugated (bs-2044R). Protein bands were developed using an HRP system.
RSC96 cells probed with LOX 1 Polyclonal Antibody, Unconjugated (bs-2044R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-2044R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

L1 rat brain lysates L2 rat kidney lysates probed with Anti BAMBI Polyclonal Antibody, Unconjugated (bs-12418R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 29kD. Observed band size:29kD.
Paraformaldehyde-fixed, paraffin embedded Human Kidney Tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with BAMBI Polyclonal Antibody, Unconjugated (bs-12418R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Lane 1: Human K562 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates probed with BAMBI Polyclonal Antibody, Unconjugated (bs-12418R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12418R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-10125R

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngeal cancer labeled Anti-CTGF Polyclonal Antibody, Unconjugated (bs-0743R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart tissue labeled Anti-CTGF Polyclonal Antibody, Unconjugated (bs-0743R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysate probed with Anti-CTGF Polyclonal Antibody (bs-0743R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.

Catalog #1: bs-0743R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled Anti-BRCA2 Polyclonal Antibody, Unconjugated (bs-1210R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by the Independent Validation Program badge 29807.\\n\\nLane 1: MCF7 cell lysate Lane 2:CAPAN1 cell lysates probed with Rabbit Anti-BRCA2 Polyclonal Antibody, Unconjugated (bs-1210R) at 1:200 for 10 hours at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BRCA2) Polyclonal Antibody, Unconjugated (bs-1210R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BRCA2) Polyclonal Antibody, Unconjugated (bs-1210R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1210R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HCCLM3 cells probed with Rabbit Anti-PEPT1 Polyclonal Antibody, Unconjugated (bs-0689R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse large intestine lysates probed with PEPT1 Polyclonal Antibody, unconjugated (bs-0689R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
SH-sy5y (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PEPT1 Antibody (bs-0689R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0689R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat hippocampus labeled with Rabbit Anti-OPN Polyclonal Antibody, Unconjugated (bs-0026R) at 1:300 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded rat shin bone labeled with Anti-Osteopontin Polyclonal Antibody, Unconjugated (bs-0026R) at 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse kidney lysates probed with (bs-00265R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Osteopontin Polyclonal Antibody(bs-0026R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0026R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat colitis tissue labeled with Anti-PDGFRA Polyclonal Antibody, Unconjugated (bs-0231R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
SH-Sy5y (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDGFRA Antibody (bs-0231R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0231R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated