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Unconjugated Primary Antibodies



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4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NUMA1) monoclonal Antibody, unconjugated (bsm-61733R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with NUMA1 monoclonal antibody, unconjugated (bsm-61733R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NUMA1 Monoclonal Antibody, Unconjugated(bsm-61733R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NUMA1 Monoclonal Antibody, Unconjugated(bsm-61733R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61733r-TR

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CYP1A2 monoclonal antibody, unconjugated (bsm-61730R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CYP1A2) monoclonal Antibody, unconjugated (bsm-61730R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-61730r-TR

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61724r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61729r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with BAF53A monoclonal antibody, unconjugated (bsm-61726R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-BAF53A antibody (bsm-61726R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-61726r

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Jab1\/COPS5 monoclonal antibody, unconjugated (bsm-61721R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The U-87MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Jab1\/COPS5 antibody (bsm-61721R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-61721r

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with IKK epsilon monoclonal antibody, unconjugated (bsm-61720R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKK epsilon Monoclonal Antibody, Unconjugated (bsm-61720R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IKK epsilon Monoclonal Antibody, Unconjugated (bsm-61720R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61720r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61717r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-90044r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90122r-50µL

Applications: WB, FCM

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90008r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90032r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90095r-50µL

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90086r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90364r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90013r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90020r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90005r-50µL

Applications: WB, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90074r-50µL

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90101r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90105r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90007r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90185r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90198r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90052r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90017r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90087r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90224r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90274r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90042r-50µL

Applications: WB, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90090r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90192r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90279r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90245r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90284r-50µL

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90268r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90186r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90246r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90137r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90269r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90263r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90213r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90281r-50µL

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90183r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90271r-50µL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90160r-50µL

Applications: WB, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90257r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90275r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated