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Unconjugated Primary Antibodies



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Catalog #1: bsm-62280r

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62277r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62278r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62262r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62276r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62272r-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62253r-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62269r-100ul

Applications: WB, IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62270r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62261r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62265r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62266r

Applications: WB, IP, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62259r-100ul

Applications: WB, FCM, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-AKR1C1\/2 antibody (bsm-62246R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488(bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with AKR1C1\/2 monoclonal antibody, unconjugated (bsm-62246R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-62246r-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62244r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLCG 2 Monoclonal Antibody, Unconjugated (bsm-62254R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLCG 2 Monoclonal Antibody, Unconjugated (bsm-62254R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLCG 2 Monoclonal Antibody, Unconjugated (bsm-62254R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLCG 2 Monoclonal Antibody, Unconjugated (bsm-62254R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62254r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MCM4) monoclonal Antibody, unconjugated (bsm-62263R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with MCM4 monoclonal antibody, unconjugated (bsm-62263R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-62263r-100ul

Applications: WB, FCM, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62256r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62257r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62239r

Applications: WB, IP, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62245r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62247r

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62242r-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62238r-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62241r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62243r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62233r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62237r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with KIF4A monoclonal antibody, unconjugated (bsm-62236R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (KIF4A) monoclonal Antibody, unconjugated (bsm-62236R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-62236r-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62225r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62235r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CD150\/SLAM monoclonal antibody, unconjugated (bsm-62229R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Molt-4(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD150\/SLAM antibody (bsm-62229R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-62229r-100ul

Applications: WB, FCM, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62224r-100ul

Applications: WB, IP

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62231r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62223r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62222r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62221r

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CHRM2 monoclonal antibody, unconjugated (bsm-62216R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHRM2 Monoclonal Antibody, Unconjugated (bsm-62216R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHRM2 Monoclonal Antibody, Unconjugated (bsm-62216R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHRM2 Monoclonal Antibody, Unconjugated (bsm-62216R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62216r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62219r

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with HLA-B monoclonal antibody, unconjugated (bsm-62217R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA-B Monoclonal Antibody, Unconjugated (bsm-62217R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA-B Monoclonal Antibody, Unconjugated (bsm-62217R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA-B Monoclonal Antibody, Unconjugated (bsm-62217R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62217r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62208r-100ul

Applications: WB, FCM, IP

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62210r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with RAD23A monoclonal antibody, unconjugated (bsm-62209R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-RAD23A antibody (bsm-62209R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RAD23A) monoclonal Antibody, unconjugated (bsm-62209R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-62209r-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MBNL1 antibody (bsm-62213R,1;100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with MBNL1 monoclonal antibody, unconjugated (bsm-62213R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-62213r-100ul

Applications: FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62202r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62214r

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62187r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Apolipoprotein D Monoclonal Antibody, Unconjugated (bsm-62203R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Apolipoprotein D Monoclonal Antibody, Unconjugated (bsm-62203R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Apolipoprotein D Monoclonal Antibody, Unconjugated (bsm-62203R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62203r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated