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Unconjugated Primary Antibodies



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Catalog #1: bs-41199R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-10879m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41176R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-23671R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33226m

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Beta catenin) Monoclonal Antibody, Unconjugated (bsm-33071M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Beta catenin) Monoclonal Antibody, Unconjugated (bsm-33071M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Beta catenin) Monoclonal Antibody, Unconjugated (bsm-33071M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Beta catenin) Monoclonal Antibody, Unconjugated (bsm-33071M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33071M

Applications: IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-20457R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10940R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7499R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-17709R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG14) Polyclonal Antibody, Unconjugated (bs-7462R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human A431 cell lysates probed with ATG14 Polyclonal Antibody, Unconjugated (bs-7462R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-7462r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Siha cell lysates probed with ARL13B Polyclonal Antibody, Unconjugated (bs-21493R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with ARL13B Polyclonal Antibody, Unconjugated (bs-21493R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-21493R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-16731R

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33423M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-23716R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21613R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-14367R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-13768R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-19774R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-19351R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-34235r

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with HDAC6 monoclonal antibody, unconjugated (bsm-52086R) at 1:5000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HDAC6 Monoclonal Antibody, Unconjugated(bsm-52086R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HDAC6 Monoclonal Antibody, Unconjugated(bsm-52086R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52086r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD38 (10A10) Monoclonal Antibody, Unconjugated (bsm-52623R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD38 Monoclonal Antibody, Unconjugated(bsm-52623R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD38 Monoclonal Antibody, Unconjugated(bsm-52623R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD38 Monoclonal Antibody, Unconjugated(bsm-52623R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52623r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52603r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of SH-Sy5Y cells with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRNP Monoclonal Antibody, Unconjugated (bsm-52505R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52505r

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

The Hela ( treated with 200J\/m2 UV-C then recovery for 30 min) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Rabbit Anti-p-Hsp27 (S78) antibody (bsm-52167R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Hela (H) cells were treated with or without UV (40 mJ\/cm2) for 30 min and anisomycin (25 \u03bcg\/mL) for 30 min , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-HSP27 (Ser78) monoclonal antibody, unconjugated (bsm-52167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSP27 (Ser78) Monoclonal Antibody, Unconjugated (bsm-52167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSP27 (Ser78) Monoclonal Antibody, Unconjugated (bsm-52167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52167r

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52204r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-52035r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Jurkat; Lane 2: K562; Lane 3: Hela lysates probed with Cdk6 (4F7) Monoclonal Antibody (bsm-52030R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody .
SW480 cells were stained with Cdk6 (4F7) Monoclonal Antibody (bsm-52030R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Cdk6 (4F7) Monoclonal Antibody (bsm-52030R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52030r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52006r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with STAT5b monoclonal antibody, unconjugated (bsm-52238R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STAT5b Monoclonal Antibody, Unconjugated (bsm-52238R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STAT5b Monoclonal Antibody, Unconjugated (bsm-52238R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STAT5b Monoclonal Antibody, Unconjugated (bsm-52238R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52238r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7369R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat lung lysates probed with Rabbit Anti-GAD65 Polyclonal Antibody, Unconjugated (bs-0325R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Hippocampus tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Hippocampus tissue lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Human U-87 MG cell lysates; Lane 9: Human Hela cell lysates; Lane 10: Human Jurkat cell lysates probed with GAD65 Polyclonal Antibody, Unconjugated (bs-0325R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0325R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14993R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10772R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-41518M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41517M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41504m

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41503m

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-34219M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Raji cell lysates probed with CD20 Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60638r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41513M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41512M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41483M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41482M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41611m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43005M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Recombinant human Beta-2-microglobulin, C-His (HEK293) probed with beta 2 Microglobulin Monoclonal Antibody, Unconjugated (bsm-43094M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HeLa cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human Raji cell lysates; Lane 5: Human PANC-1 cell lysates probed with beta 2 Microglobulin Monoclonal Antibody, Unconjugated (bsm-43094M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-43094m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated