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Unconjugated Primary Antibodies



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Catalog #1: bsm-60636r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41359r

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41357R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41343R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-22114R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (bsm-41495M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (bsm-41495M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (bsm-41495M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (THRB) Monoclonal Antibody, Unconjugated (bsm-41495M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-41495m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41061R

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Recombinant mouse Trmt2b protein probed with TRMT2B Polyclonal Antibody, Unconjugated (bs-49205R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant mouse Trmt2b protein, N-His(bs-49205P) probed with TRMT2B Polyclonal Antibody, Unconjugated (bs-49205R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-49205R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-34218m

Applications: WB, ELISA, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-24915R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Recombinant human IL-6 protein (bs-10807P); Lane 2: Recombinant human IL-6 protein (bs-41177P) probed with IL-6 Polyclonal Antibody, Unconjugated (bs-41177R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HUVEC cell lysates; Lane 2: Human HeLa cell lysates probed with IL-6 Polyclonal Antibody, Unconjugated (bs-41177R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-41177R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25220R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-25219R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25327R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41202R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2294R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-31218m

Applications: WB, ELISA, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10807R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-20758R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates probed with GFAP Polyclonal Antibody, Unconjugated (bs-42001R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with GFAP polyclonal antibody, unconjugated (bs-42001R) at 1:20000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed U87-MG (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GFAP) polyclonal Antibody, unconjugated (bs-42001R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bs-42001r

Applications: WB, ELISA, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41377r

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-20429r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60633r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-16616R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41371r

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41293R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43033r

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-42008R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-14287R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-22876R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41364R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-41346r

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human 293T cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Mouse 4T1 cell lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Hamster CHO cell lysates; Lane 7: Rat Cerebrum tissue lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-41373R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-41373R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-41373R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-41373R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-41373R

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-2864M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Rat Testis tissue lysates probed with SRC Monoclonal Antibody, Unconjugated (bsm-33435M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human HepG2 cell lysates probed with SRC Monoclonal Antibody, Unconjugated (bsm-33435M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33435M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33434M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-41140r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41393R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-41355r

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Rat Breast tissue lysates; Lane 4: Human SH-SY5Y cell lysates; Lane 5: Human A431 cell lysates probed with IL-20R alpha Polyclonal Antibody, Unconjugated (bs-41354R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant human IL-20R alpha protein probed with IL-20R alpha Polyclonal Antibody, Unconjugated (bs-41354R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-41354r

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41398r

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-21184R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-39018R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60736r

Applications: IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (bsm-60735R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD8) Monoclonal Antibody, Unconjugated (bsm-60735R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD8 monoclonal antibody, unconjugated (bsm-60735R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60735r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43073M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-23765R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-22163R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated