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Unconjugated Primary Antibodies



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Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-PTPN11(Tyr511)) Polyclonal Antibody, Unconjugated (bs-39021R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-PTPN11(Tyr511)) Polyclonal Antibody, Unconjugated (bs-39021R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-39021r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7357r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (bs-49099R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (bs-49099R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Testis tissue lysates probed with H2-Q10 Polyclonal Antibody, Unconjugated (bs-49099R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-49099r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43017R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43012R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (bs-41348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (bs-41348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (bs-41348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (bs-41348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-41348r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (bs-41347R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (bs-41347R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-41347r

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41324r

Applications: WB

Reactivity: Bacteria

Conjugate: Unconjugated

Catalog #1: bs-41320R

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-34106R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-34105r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2854R

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-2833R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG5\/APG5L) Polyclonal Antibody, Unconjugated (bs-25013R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG5\/APG5L) Polyclonal Antibody, Unconjugated (bs-25013R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with ATG5\/APG5L Polyclonal Antibody, Unconjugated (bs-25013R) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human PANC-1 cell lysates; Lane 2: Human U87MG cell lysates probed with ATG5\/APG5L Polyclonal Antibody, Unconjugated (bs-25013R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-25013r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-25008R

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-24945R

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-22682R

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21419R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IRF-1) Polyclonal Antibody, Unconjugated (bs-21318R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IRF-1) Polyclonal Antibody, Unconjugated (bs-21318R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-21318r

Applications: IHC-P, IHC-F, IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-20168R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-23809R

Applications: WB, IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Sample:\r\nTestis (Mouse) Lysate at 40 ug\r\nPrimary: Anti- TLR9 (bs-23640R) at 1\/1000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 113 kD\r\nObserved band size: 120 kD
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human HeLa cell lysates probed with TLR9 Polyclonal Antibody, Unconjugated (bs-23640R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-23640R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-14614R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-20498R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-19059R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-16779R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-41516M

Applications: WB

Reactivity: Mouse, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41637M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41630M

Applications: WB, ELISA

Reactivity: Mouse, Virus, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41615M

Applications: WB, ELISA

Reactivity: Mouse, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41632M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-41634M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41635M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41631M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41627M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41636M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-41621M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41476M

Applications: WB, ELISA

Reactivity: Mouse, Virus, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41519R

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bs-24426r

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-24425r

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-41407r

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60088m

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Arabidopsis thaliana\r\nProtein loading quantity: 5 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Rice\r\nProtein loading quantity: 0.008 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa

Catalog #1: bsm-60435m

Applications: WB

Reactivity: Arab

Conjugate: Unconjugated

Catalog #1: bsm-60569m

Applications: WB, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60172m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-60072r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: (-) JK, (+) JK+Etopside (100ug\/ml, 24hr) +Calyculin A (100nM, 1hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: (-) C2C12, (+) C2C12+Nocodazole (100ng\/ml, 18hr) +Calyculin A (100nM, 1hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: Mouse spleen, Mouse liver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:200\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bs-60074R\r\n\r\n

Catalog #1: bs-60074r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated