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Unconjugated Primary Antibodies



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Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PER-3 Polyclonal Antibody, Unconjugated (bs-4498R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PER-3 Polyclonal Antibody, Unconjugated (bs-4498R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4498R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZNF775 Polyclonal Antibody, Unconjugated (bs-4382R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZNF775 Polyclonal Antibody, Unconjugated (bs-4382R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4382R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4318R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACE2 Polyclonal Antibody, Unconjugated (bs-41410R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACE2 Polyclonal Antibody, Unconjugated (bs-41410R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACE2 Polyclonal Antibody, Unconjugated (bs-41410R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACE2 Polyclonal Antibody, Unconjugated (bs-41410R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-41410R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41239R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41197M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-IRS1 (Tyr632) ) polyclonal Antibody, Unconjugated (bs-8707R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-8707R

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-41196M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF2 Monoclonal Antibody, Unconjugated(bsm-34016R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF2 Monoclonal Antibody, Unconjugated(bsm-34016R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF2 Monoclonal Antibody, Unconjugated(bsm-34016R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FGF2 Monoclonal Antibody, Unconjugated(bsm-34016R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-34016r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PTEN) monoclonal Antibody, unconjugated (bsm-33319M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed NIH\/3T3(M) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PTEN) monoclonal Antibody, unconjugated (bsm-33319M) 1:50, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTEN Monoclonal Antibody, Unconjugated(bsm-33319M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTEN Monoclonal Antibody, Unconjugated(bsm-33319M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33319m

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33245M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Transferrin(Serum Loading Control) Monoclonal Antibody(bsm-33243M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with Transferrin monoclonal antibody, unconjugated (bsm-33243M) at 1:5000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33243m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33224M

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33221M

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33201M

Applications: WB

Reactivity: Goat, Cow

Conjugate: Unconjugated

Catalog #1: bsm-33198M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H2B Monoclonal Antibody, Unconjugated (bsm-33174M) at 1:800 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H2B Monoclonal Antibody, Unconjugated (bsm-33174M) at 1:800 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H2B Monoclonal Antibody, Unconjugated (bsm-33174M) at 1:800 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33174M

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:800 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Aquaporin 4 Monoclonal Antibody, Unconjugated (bsm-33068M) at 1:800 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33068M

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-30105M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0514M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9528R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-8711R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7492R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7451R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-55141R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: PRKAR2A knockout (KO) HeLa cell lysates probed with PRKAR2A Polyclonal Antibody, Unconjugated (bs-55176R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates; Lane 3: SW480 cell lysates; Lane 4: LO2 cell lysates; Lane 5: Mouse Lung lysates; Lane 6: Rat skeletal muscle lysates probed with PRKAR2A Polyclonal Antibody, Unconjugated (bs-55176R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55176R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55209R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human thyroid cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: SW620 cell lysates probed with SIRT3 Polyclonal Antibody, Unconjugated (bs-55199R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55199R

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SCD) polyclonal Antibody, Unconjugated (bs-55193R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: H460 cell lysates; Lane 2: Mouse Liver lysates probed with SCD Polyclonal Antibody, Unconjugated (bs-55193R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: SCD knockout (KO) HeLa cell lysates probed with SCD Polyclonal Antibody, Unconjugated (bs-55193R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55193R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human U2OS cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Mouse PC-12 cell lysates probed with RHOC Polyclonal Antibody, Unconjugated (bs-55187R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: RHOC knockout (KO) 293T cell lysates probed with RHOC Polyclonal Antibody, Unconjugated (bs-55187R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (RHOC ) polyclonal Antibody, Unconjugated (bs-55187R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55187R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: BT474 cell lysates; Lane 2: SKOV3 cell lysates; Lane 3: U251MG cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat Brain lysates probed with PTPN1 Polyclonal Antibody, Unconjugated (bs-55182R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: PTPN1 knockout (KO) HeLa cell lysates probed with PTPN1 Polyclonal Antibody, Unconjugated (bs-55182R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PTPN1 Polyclonal Antibody, Unconjugated (bs-55182R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PTPN1 Polyclonal Antibody, Unconjugated (bs-55182R) at 1:100 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55182R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: PDCD4 knockout (KO) HeLa cell lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Mouse Thymus lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55166R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Jurkat cell lysates; Lane 2: Human Jurkat cell lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat C6 cell lysates; Lane 4: Mouse testis lysates; Lane 5: Mouse brain lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: PARP1 knockout (KO) HeLa cell lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-55164R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PARP1) polyclonal Antibody, Unconjugated (bs-55164R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55164R

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nP21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nP21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nP21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: P21 knockout (KO) HeLa cell lysates probed with P21 Polyclonal Antibody, Unconjugated (bs-55160R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55160R

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human 293T cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Mouse Thymus lysates; Lane 6: Mouse Testis lysates; Lane 7: Rat Thymus lysates; Lane 8: Rat Testis lysates probed with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: HDAC1 knockout (KO) 293T cell lysates probed with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HDAC1) polyclonal Antibody, Unconjugated (bs-55093R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:100 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55093R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21401R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Mouse Brain lysates; Lane 4: Mouse Spleen lysates; Lane 5: Rat Brain lysates; Lane 6: Rat Spinal node lysates probed with ERK2 Polyclonal Antibody, Unconjugated (bs-55069R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: ERK2 knockout (KO) HeLa cell lysates probed with ERK2 Polyclonal Antibody, Unconjugated (bs-55069R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55069R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-34021R

Applications: WB, ELISA

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-34012R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-33411R

Applications: WB, ICC/IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-24359R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-24356R

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-22318R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-23785R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-23551R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Goat, Cow

Conjugate: Unconjugated

Catalog #1: bs-23205R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-23173R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-22547R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated