ADRB3 Polyclonal Antibody

Mouse intestine lysate 30ug, probed (bs-10921R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:10000 90min in 37˚C.

Formalin-fixed and paraffin embedded rat adrenal gland labeled with Anti-ADRB3 Polyclonal Antibody, Unconjugated (bs-10921R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human kidney labeled with Anti-ADRB3 Polyclonal Antibody, Unconjugated (bs-10921R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: Mouse Urinary bladder tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Stomach tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human U-2 OS cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with ADRB3 Polyclonal Antibody, Unconjugated (bs-10921R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Urinary bladder tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Stomach tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human U-2 OS cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with ADRB3 Polyclonal Antibody, Unconjugated (bs-10921R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Horse
Rabbit

Overview
Catalog #	bs-10921R
Product Name	ADRB3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ADRB3
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	155
Swiss Prot	P13945
Subcellular location	Cell membrane
Synonyms	BETA3AR; Beta-3 adrenergic receptor; Beta-3 adrenoreceptor; Beta-3 adrenoceptor; ADRB3; ADRB3R; B3AR
Background	Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200