Neuroglycan C Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Neuroglycan Polyclonal Antibody, Unconjugated (bs-11323R) at 1:300 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n$

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Neuroglycan Polyclonal Antibody, Unconjugated (bs-11323R) at 1:300 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n

$Human U251 cells probed with Neuroglycan C Polyclonal Antibody, unconjugated (bs-11323R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n$

Human U251 cells probed with Neuroglycan C Polyclonal Antibody, unconjugated (bs-11323R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n

$Mouse cerebrum lysates probed with Neuroglycan C Polyclonal Antibody, unconjugated (bs-11323R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n$

Mouse cerebrum lysates probed with Neuroglycan C Polyclonal Antibody, unconjugated (bs-11323R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Pig

Overview
Catalog #	bs-11323R
Product Name	Neuroglycan C Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Neuroglycan C
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10675
Swiss Prot	O95196
Subcellular location	Cytoplasm, Cell membrane
Synonyms	NGC; Chondroitin sulfate proteoglycan 5; Acidic leucine-rich EGF-like domain-containing brain protein; Neuroglycan C; CSPG5; CALEB
Background	May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200