CWC22 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-11464R
Product Name	CWC22 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CWC22
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	57703
Subcellular location	Nucleus
Synonyms	CWC22; CWC22 spliceosome associated protein homolog S. cerevisiae; CWC22 spliceosome associated protein homolog; CWC22_HUMAN; E4GL; fSAPb; Functional spliceosome associated protein b; KIAA1604; NCM; Nucampholin homolog; Pre-mRNA-splicing factor CWC22 homolog.
Background	Spliceosomes are multi-protein complexes that are composed of snRNPs (small nuclear ribonucleoproteins) and a variety of associated protein factors, all of which work in concert to regulate the splicing of pre-mRNA, a critical step in the post-transcriptional regulation of gene expression. CWC22 (CWC22 spliceosome-associated protein), also known as NCM, fSAPb or EIF4GL, is a 908 amino acid nuclear protein and component of the spliceosome C complex. CWC22 is associated with the spliceosome prior to catalytic steps and remains associated throughout the reaction. Belonging to the CWC22 family, CW22 contains one MI domain and a MIF4G domain. The gene encoding CWC22 maps to human chromosome 2q31.3. As the second largest human chromosome, chromosome 2 makes up approximately 8% of the human genome and contains 237 million bases encoding over 1,400 genes.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200